肉苁蓉及淫羊藿对小鼠早期胚胎体外发育的影响

Effects of Cistanche and Epimedium on Development of Early Mouse Embryo

研究肉苁蓉及淫羊藿对小鼠早期胚胎体外发育的影响。试验选取6～8周龄昆明小鼠早期胚胎,以不添加中药培养液组为空白对照,分以下试验组:I组:早期胚胎在纯培养液M16+5%FBS(M1)与M16+5%FBS+EDTA+牛磺酸(M2)中的体外发育情况;Ⅱ组:早期胚胎添加至中药体积浓度为0.1%的M1与M2培养液中。Ⅲ组:早期胚胎添加至不同体积浓度中药成分的培养液中。Ⅳ组:将早期胚胎加入中药不同组合体积浓度培养液中。试验结果:(1)M2液优于M1液,特别是在克服2细胞发育阻滞上。(2)添加0.1%肉苁蓉桑葚胚率与囊胚率高于0.1%淫羊藿组,差异极显著(P<0.01),中药组桑葚胚率与囊胚率显著高于培养液空白对照,差异极显著(P<0.01)。(3)高浓度中药会对胚胎活力产生副作用,抑制胚胎后阶段发育,与其他组对比差异极显著(P<0.01)。(4)添加高浓度中药组合培养液对早期胚胎成活率、2细胞发育率,与低浓度组合中药和空白组相比有显著差异性(P<0.05),与低浓度组合中药和空白对照相比桑葚胚及囊胚发育率差异极显著(P<0.01)。在小鼠胚胎体外培养液中添加一定浓度EDTA、牛磺酸能克服2细胞阻滞;0.1%肉苁蓉能有效促进胚胎体外发育,同时添加低浓度中药及低浓度组合中药也有促进作用。

A study on the influence of Cistanche and Epimedium on development of early mouse embryos in vitro was conducted.Early embryos of 6-8 week-old KM mice were selected for test.A group of no medicine was taken as the blank control,The others were divided into the following groups,Group I：early embryos in medium of M1 and M2.GroupⅡ：early embryos in the medium of 0.1% of M1 and M2.GroupⅢ：early embryos in medium of Chinese medicines of different concentrations.GroupⅣ： early embryos in medium of different combinations of Chinese medicines.The results showed that,（1）M2 was better than M1,especially in overcoming the block of 2-cells.（2）Addition 0.1% Cistanche was better than that of 0.1% Epimedium in Morula and blastula（P〈0.01）,the rates of Morula and blastula in the medicine groups were higher than that in the control（P〈0.01）.（3）High concentration of Chinese medicines had bad effects on viability of embryo,and restrained the rates of developent of Morula and blastula（P〈0.01）.（4）Compared with the control,there was significant difference betwwen addition of portfolio of high concentration of Chinese medicines and that of low concentration in survival rate,development rate（P0.05）.At the same time,the development rates of Morula and blastula compared with those in the low concentration groups and the control were significantly different（P〈0.01）.It means with high concentration of Chinese medicine will inhibi the development.Conclusion： medium added with EDTA,taurine,0.1% Cistanche can overcome the block of 2-cells in vitro,and addition of low concentrations or combination of medicines has good effects.