摘要
目的:建立同时测定小儿清热止咳口服液中盐酸麻黄碱、盐酸伪麻黄碱和苦杏仁苷含量的方法。方法:采用高效液相色谱法。色谱柱为Platisil ODS柱,流动相为乙腈-0.1%磷酸溶液(含0.1%三乙胺)=5∶95,流速为1.0mL·min-1,检测波长为215nm,进样量为10μL,柱温为30℃。结果:盐酸麻黄碱进样量在0.146~4.85μg范围内与峰面积积分值呈良好的线性关系(r=0.9999),平均加样回收率为99.4%,RSD=1.0%;盐酸伪麻黄碱进样量在0.157~5.23μg范围内与峰面积积分值呈良好的线性关系(r=0.9999),平均加样回收率为99.6%,RSD=1.7%;苦杏仁苷进样量在0.297~9.90μg范围内与峰面积积分值呈良好线性关系(r=0.9999),平均加样回收率为98.9%,RSD=0.7%。结论:该方法简便、灵敏、准确,可用于小儿清热止咳口服液的质量控制。
OBJECTIVE: To establish the method for the content determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and amygdalin in Xiao’er qingre zhike oral solution. METHODS: HPLC method was adopted. The determination was performed on Platisil ODS column with mobile phase consisted of acetonitrile-0.1%phosphoric acid solution (containing 0.1% triethylamine, 5 ∶ 95) at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 215 nm and injection volume was 10 μL. The column temperature was 30 ℃. RESULTS: The linear range of ephedrine hydrochloride was 0.146~4.85 μg (r=0.999 9) with an average recovery of 99.4% (RSD=1.0%). The linear range of pseudoephedrine hydrochloride was 0.157~5.23 μg (r=0.999 9) with an average recovery of 99.6% (RSD=1.7%). The linear range of amygdalin was 0.297~9.90 μg (r=0.999 9) with an average recovery of 98.9% (RSD=0.7%) . CONCLUSION: The method is simple, sensitive and accurate, which can be used for the quality control of Xiao’er qingre zhike oral solution.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第44期4212-4214,共3页
China Pharmacy