瑞香狼毒药效组分对裸鼠肝癌移植瘤的抑制及其机制

Antitumor activity of active components alignment isolated from Stellera chamaejasme L.in nude mice bearing subcutaneous BEL-7402 hepatoma

目的:评价瑞香狼毒药效组分抑制人肝癌细胞株BEL-7402裸鼠皮下移植瘤作用的强度,并探讨其机制.方法:建立人肝癌BEL-7402裸小鼠皮下移植瘤模型研究瑞香狼毒药效组分Zp1111抗肿瘤作用与对荷瘤裸鼠体质量与免疫器官的影响,免疫组织化学法检测用药前后BEL-7402皮下移植瘤组织Bcl-2与Bax阳性表达的变化.结果:Zp1111对裸小鼠皮下移植瘤BEL-7402具有较强的抑制作用,187.5mg/kg、250mg/kg(折合成生药量)剂量时对移植瘤的相对肿瘤增殖率T/C小于60%;该组分能显著上调BEL-7402移植瘤组织Bax基因的表达,但对Bcl-2的表达影响较弱.结论:瑞香狼毒药效组分在体内对人肝癌细胞BEL-7402裸鼠移植瘤具有较强的抑制作用并呈现一定的剂量依赖关系,该组分具有一定的诱导凋亡作用,可能与上调Bax有关.

AIM： To assess the antitumor activity of the ef- fective extracts of S. chamaejasme L. （ScL） in a murine model of hepatocellular carcinoma and to explore the possible mechanisms involved. METHODS： A tumor transplantation model was generated in nude mice to study the antitumor activity of the active components alignment Zp1111 in vivo. Immunohistochemistry （IHC） was employed to investigate the expression of Bcl-2 and Bax in tumor tissue. RESULTS： Zp1111 exhibited strong antitumoractivity in BEL7402-bearing nude mice, with the relative tumor proliferation rate （T/C） less than 60% at a dose of 187.5 or 250 mg/kg. Treatment with Zp1111 up-regulated Bax expression and slightly down-regulated Bcl-2 expression in tu- mor tissue. CONCLUSION： The active components align- ment of ScL significantly reduces BEL-7402 proliferation in a dose-dependent manner in vivo possibly by up-regulating Bax expression to induce cell apoptosis.