rhTGF-β1对大鼠髁突软骨细胞SZP表达的影响

Effects of rhTGF-β1 on the SZP Expression and Secretion in the Chondrocytes Derived from Rat Condyle Cartilage

目的:观察rhTGF-β1对大鼠髁突软骨细胞SZP表达的影响。方法:酶消化法获取大鼠髁突软骨细胞,甲苯胺蓝和II型胶原染色鉴定。细胞培养液中分别加入10μg/L、20μg/L rhTGF-β1后,不同时间点应用RT-PCR和ELISA法检测SZP mRNA和蛋白的表达。结果:加入10μg/L、20μg/L rhTGF-β1 24h后髁突软骨细胞中SZP mRNA及蛋白表达均增高,且具有统计学差异(P<0.05)。加入10μg/L rhTGF-β1 24h、48h、72h后SZP mRNA及蛋白表达逐渐增强,且具有统计学差异(P<0.05)。结论:rhTGF-β1能够增强大鼠髁突软骨细胞SZP的合成和分泌,并具有时间依赖性。

Objective： To investigate the effects of transforming growth factor-β1（TGF-β1） on the expression and secretion of superficial zone protein（SZP） in the articular chondrocytes derived from rat condyle.Methods： The chondrocytes were isolated from rat mandibular condyle by enzyme digestion and phenotypically determined by toluidine blue staining and collagen II immunohistochemistry staining.After exposed to rhTGF-β1（10 and 20ng/ml） for indicated time course,the SZP mRNA expression and secretion by chondrocytes were assessed by RT-PCR and ELISA,respectively.Results： Treatment of chondrocytes with rhTGF-β1 resulted in significant enhancement in the mRNA expression and secretion of SZP as compared with control（P0.05）.Moreover,rhTGF-β1（10 ng/ml） exposure remarkably induced the expression of SZP over a 72 hour time course.Conclusion： TGF-β1 can enhance the SZP expression and secretion in cultured condylar chondrocytes in a time-dependent manner.