广玉兰幼果水提物对动物血管和离体心脏的影响

The effects of aqueous extract of magnolia young fruit on animal blood vessel and isolated heart

目的探讨广玉兰幼果水提物对动物血管和离体心脏作用的影响。方法利用离体血管灌流法,用locke液灌注离体兔耳血管,观察给药前后兔耳灌流量的变化。采用动脉血压直接测定法,记录广玉兰幼果水提物对麻醉狗血压的影响。应用离体心脏灌流法(langendorff法),以恒流方式,在恒温恒压条件下,用Rin-ger-locke液灌注豚鼠离体心脏,观察给药前后豚鼠心脏灌流量和心功能的变化,并采用BL-420E生物机能实验系统,测量计算给药前豚鼠离体心脏收缩幅度、张力、频率及在广玉兰幼果水提物、美托洛尔、维拉帕米作用下对豚鼠离体心脏的影响。结果广玉兰幼果水提物使离体兔耳血管的灌流量明显减少并引起麻醉狗血压的升高;广玉兰幼果水提物使豚鼠离体心脏的收缩幅度显著增强,但收缩频率、张力和灌流量无明显变化,且收缩作用可被美托洛尔、维拉帕米所拮抗。结论广玉兰幼果水提物具有收缩血管、兴奋心脏、升高血压的作用;其作用机制是通过β受体和Ca2+通道产生的。

Objective In the present study we investigated the effects of aqueous extract of magnolia young fruits on the blood vessels and isolated heart in the animals.Methods The ear irrigate flow rate was recorded by the method of the isolated rabbit ear blood vessel.The effects of aqueous extract of magnolia young fruits on blood pressure were observed in anesthetizing dog.According to the method of langendorff the isolated heart of the cavy was poured with Ringer-locke liquid using constant current way.All the procedures were under the condition of constant temperature and pressure.The coronary artery flow rate and heart function were observed.And the contraction amplitude,tension and frequency of the cavy isolated heart were measured by BL-420E biological function system.Then we explored the effects of aqueous extract of magnolia young fruits,Metoprolol and verapamil on the cavy isolated heart.Results The aqueous extract of magnolia young fruits decreased the irrigate flow rate of the isolated rabbit ear blood vessel and strengthened the contraction of blood vessel in dogs.The contraction amplitude of the cavy isolated heart significantly increased by the aqueous extract of magnolia young fruits.However,the contraction frequency,tension and irrigate flow rate were not changed.The function of the contraction was inhibited by Metoprolol and Verapamil.Conclusion The aqueous extract of magnolia young fruits could contract blood vessel,raise blood pressure and excite heart by β-receptor and Ca2＋ channel.