水生生物近缘种和产地的分子生物学判别

The Identification of Closely Related Species and Origin of Aquatic Organisms by Molecular Approaches

随着分子生物学技术的发展,渔业生物近缘种及产地的判别由形态学深入到了蛋白质和DNA水平。本文综述了目前已经应用于水生生物的相关分子生物学技术(包括色谱、免疫学判别以及蛋白的电泳、DNA的序列分析、PCR-RFLP、RAPD、AFLP、SNP、SSRI、SSR、Real-Time PCRs、pecies-specific primer PCR和PCRlab-on-a-chip等)以及它们各自的优缺点;在把握了这些研究进展的基础上,展望了相关技术的发展趋势。

With the development of molecular biology,the approaches for closely related species and origin identification of aquatic organisms has developed from morphological way to molecular level.The article summarizes current protein and DNA techniques that have been applied in corresponding identification（e.g.,chromatography,immunology identification,and protein electrophoresis,DNA sequence analysis,PCR-RFLP,RAPD,AFLP,SNP,SSR,ISSR,Real-Time PCR,species-specific primer PCR,PCR lab-on-a-chip） and makes comparison of their advantages and disadvantages.On the basis of the advances above,the future development tendency of the approaches is expected in the present article.