摘要
目的:观察曲马多预处理对氯胺酮抗抑郁作用的影响。方法:32只Wistar大鼠随机分为生理盐水组(S组)、曲马多组(T组)、氯胺酮组(K组)、曲马多+氯胺酮组(T+K组)。药物预处理前1天大鼠强迫游泳15 min;预处理当天各组分别腹腔注射1 mL容积的生理盐水、曲马多5 mg.kg-1、生理盐水、曲马多5 mg.kg-1;30 min后,各组分别注射生理盐水、生理盐水、氯胺酮10 mg.kg-1、氯胺酮10 mg.kg-1;再经30 min后,行强迫游泳试验并记录其不动时间,取海马组织测定脑源性神经营养因子(BDNF)及酪氨酸受体激酶B(TrkB)的含量。结果:与S组相比,K组、T+K组强迫游泳试验不动时间减少,海马BDNF及TrkB表达增加(P<0.05);与K组相比,T+K组强迫游泳试验不动时间减少,海马BDNF及TrkB表达增加(P<0.05)。结论:曲马多预处理能增强氯胺酮的抗抑郁作用,这可能与大鼠海马BDNF及TrkB表达上调有关。
Objective： To observe the effects of tramadol pretreatment on ketamine-induced antidepressant action.Methods： Thirty-two Wistar rats were randomly divided into saline group（S group）,tramadol group（T group）,ketamine group（K group） and tramadol＋ketamine group（T＋K group）.One day before drug pretreatment,rats were insulted in the forced swimming test（FST） for 15 min.At the day of drug pretreatment,rats were intraperitoneally injected with 1 ml of saline,tramadol 5 mg·kg-1,saline and tramadol 5 mg·kg-1,respectively;Thirty minutes later,rats were intraperitoneally injected with saline,saline,ketamine 10 mg·kg-1 and ketamine 10 mg·kg-1,respectively.Another 30 min later,FST was conducted and the immobility time was recorded.Rat hippocampus was harvested and the levels of brain-derived neurotrophic factor（BDNF） and tyrosine receptor kinases B（TrkB） were detected.Results： Compared with S group,the immobility time of rats receiving FST decreased and the expression of hippocampal BDNF and TrkB increased in K and T＋K groups（P0.05）.Compared with K group,the immobility time decreased and the expression of hippocampal BDNF and TrkB increased in T＋K group.Conclusion： Tramadol pretreatment can reinforce the antidepressant action of ketamine,which may be attributed to the increase in the expression of hippocampal BDNF and TrkB.
出处
《药学与临床研究》
2011年第5期426-428,共3页
Pharmaceutical and Clinical Research
