摘要
建立应用纳升级超高效液相色谱-质谱(nanoUPLC Q-TOF MS/MS)联用技术研究大鼠同位肝移植术后不同时间差异表达蛋白质的蛋白质组学方法。建立10组大鼠肝移植动物模型后,随机分成术后3天组(n=5)和术后7天组(n=5)。分别于术后3天和7天处死5只受体,取肝组织,提取总蛋白,Trypsin酶解后,使用Waters公司独家专利MSeTM无标记定量技术,利用nanoUPLC-MS/MS技术对酶解后的肽段进行分析,数据库检索鉴定蛋白质,采用生物信息学工具对所鉴定的蛋白质进行功能分类。7天组与3天组相比,两样品中同时存在的大部分蛋白上调,其中10个蛋白点表达水平明显上调,比值变化在8.5倍以上。结合数据库搜索得到鉴定,这些明显上调的蛋白的分子功能主要与细胞骨架、离子结合、氧化应激反应、能量代谢等相关。
Novel approaches for the qualitative and quantitative proteomics analysis by nanoscale LC/MS was applied to the study of protein expression respond in organ transplant. In this experiment nanoUPLC Q-TOF-based LC-MS/MS platforms was evaluated for shotgun proteomics by compare the protein level after the liver transplant between Sprague Dawley（SD） and Wistar. A brand new technology label-free LC/MS called MSeTM from a nanoUPLC and Q-TOF （Waters） was applied attempt to establish a scientific model to for the liver transplant between Sprague Dawley（SD） and Wistar. The result is most of the protein found in the model are up-regulated between the 3 days sample and the 7 days sam- ple after transplant. The novel LC/MS technology provides a fast and reliable research platform in the modeling of transplant proteomic research, provides the opportunities for discovery of new potential biomarkers.
出处
《质谱学报》
EI
CAS
CSCD
2011年第5期314-320,共7页
Journal of Chinese Mass Spectrometry Society
