摘要
从粳稻中花11转基因后代中发现了一个半矮化小穗突变体,表现为植株半矮化、生长势弱、半包茎穗、穗型变小等特点,将其命名为sdsp2(semidwarfandsmallpanicle2)。遗传分析显示,该突变体表型受1对隐性核基因控制。以sdsp2突变体为母本与龙特甫B杂交构建F2分离群体,将该基因定位在水稻第6染色体的RH6-32和RH6-40之间的116kb的物理距离内。通过水稻基因组注释系统在此区域预测到14个开放阅读框,未发现与已报道穗型发育相关基因的同源基因。
A semi-dwarf and small panicle mutant was obtained from a japonica rice variety Zhonghua 11 through T-DNA transformation.The mutant,characterized by semi-dwarf plant,abnormal small and semi-enclosed panicle,and poor growth potential was designated as sd-sp2(semi-dwarf and small panicle 2).Genetic analysis indicated that the mutant was controlled by a single recessive gene.A genetic linkage map was constructed using an F2 mapping population derived from a cross between the sd-sp2 mutant and an indica variety Longtefu B on chromosome 6.The gene was finally located on a 116-kb region between the STS markers RH6-32 and RH6-40,where 14 ORFs were predicted based on Rice Genome Annotation.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2011年第6期677-680,共4页
Chinese Journal of Rice Science
基金
浙江省自然科学基金重点项目(Z3110509)
国家转基因生物新品种科技重大专项(2008ZX08010-002)
关键词
水稻
半矮化
小穗
突变体
基因定位
rice
semi-dwarf
small panicle
mutant
gene mapping