巴马长寿人群基因与环境交互作用分析

Influence of gene-environment interactions on longevity in Bama county

目的探讨基因-环境、基因与基因交互作用对长寿人群的影响。方法选取广西巴马瑶族自治县5个乡镇,按百岁老人比例分为长寿区和非长寿区,采用单纯病例研究法,对其中≥90岁老年人189人进行调查,采集血液样品,检测CSF1PO A9等23个等位基因或基因型,以居住长寿区或非长寿区为环境暴露因素,采用交叉乘积比值比(COR)分析基因-环境、基因-基因交互作用。结果居住巴马长寿区分别与等位基因D7S820 A12(COR=0.353,95%CI=0.142～0.882,χ2=5.287,P=0.021)、等位基因TH01 A10(COR=0.111,95%CI=0.038～0.328,χ2=17.439,P=0.000)及基因型TH01 A9-10(COR=0.062,95%CI=0.014～0.283,χ2=21.009,P=0.001)存在负相乘模型交互作用;等位基因D7S820 A12与TH01 A10的基因-基因交互作用分析显示,两等位基因无交互作用(COR=1.536,95%CI=0.543～4.345,χ2=0.279,P=0.597)。结论携带等位基因D7S820 A12、TH01 A10、基因型TH01 A9-10可能会减弱居住在巴马长寿区人群获得长寿的可能性;巴马不同地理环境居住地与基因的交互作用可能影响巴马人的长寿,可能是造成巴马长寿人群在空间分布不均的原因之一。

Objective To assess the influence of gene-environment and gene-gene interactions on longevity in Bama county. Methods Both gene-environment and gene-gene interactions among 189 longevous individuals（≥90 years old）from both longevity and non-longevity regions were evaluated according to case-only approach using cross-product odd ratio（COR）.Blood samples were collected from all subjects.Alleles and genotypes of 23 loci,such as CSF1PO A9,were analyzed. Results Significant negative interactions between longevity region and D7S820 A12（COR=0.353,95% confidence interval：0.142-0.882,χ2=5.287,P=0.021）,longevity region and TH01 A10（ COR=0.111,95%CI：0.038-0.328,χ2=17.439,P=0.000）,longevity region and TH01 A9-10（ COR=0.062,95%CI：0.014-0.283,χ2=21.009,P=0.001） were showed when ten alleles and thirteen genotypes were analyzed.There was no significant gene-gene interaction between D7S820 A12 and TH01 A10（ COR=1.536,95%CI：0.543-4.345,χ2=0.279,P=0.597）. Conclusion The results suggest that the population from longevity region carrying the alleles of D7S820 A12,TH01 A10,and TH01 A9-10 genotype may reduce longevity.Gene-enviroment interactions between longevity region and genes may influence longevity in Bama,which is probably one of the reasons that there is a nonuniform distribution of longevous population in Bama.