麦芽酚铝对大鼠学习记忆能力影响

Effect of aluminum-maltolate on learning and memory in rats

目的探讨麦芽酚铝[Al(mal)3]的神经毒性。方法经行为学筛选后,40只雄性SD大鼠按体重随机分为5组,对照组、麦芽酚组、Al(mal)3 0.27、0.54、1.08 mg/kg染毒组,采用亚慢性腹腔注射方式染毒60 d。通过水迷宫实验、跳台试验测定大鼠神经行为学改变,石墨炉原子吸收光谱法测定大鼠脑皮质铝含量。结果亚慢性腹腔注射Al(mal)3染毒可导致大鼠morris水迷宫实验潜伏期逐渐延长,穿越原平台位置次数明显减少(P<0.05);Al(mal)3染毒组潜伏期明显缩短,错误次数1和错误次数2明显增加,与对照组、麦芽酚组比较,差异具有统计学意义(P<0.05);Al(mal)3 0.27、0.54、1.08 mg/kg染毒组脑皮质铝含量分别为(43.16±1.54)、(53.38±3.85)、(89.20±14.45)ng/g,与对照组和麦芽酚组脑皮质铝含量(19.50±4.12)、(21.63±4.05)ng/g比较,差异有统计学意义(P<0.05)。结论亚慢性Al(mal)3染毒对大鼠神经系统有损伤作用。

Objective To explore the neurotoxicity of aluminum-maltolate（Al3）. Methods Behavior screening on two month old Sprague Dawley（SD） male rats was carried out with Morris water maze and open-field test.After the screening,forty SD male rats were divided randomly into five groups：normal saline group,maltolate group,0.27,0.54,and 1.08 mg/kg Al（mal）3 treated groups.The rats in the five groups received corresponding reagent of 200 μl/day via intraperitoneal injection for two months.After the treatments,the Morris water maze test and step-down test were performed to examine learning and memory abilities of the rats.Aluminum concentration in rat neocortex was determined by graphite furnace atomic absorption spectrometry. Results The average escape latency of water maze test prolonged obviously and the average numbers of crossing the platform site decreased significantly in Al（mal）3 groups（P0.05 for all）.In step down test,the results of analysis of variance（ANOVA） showed significant differences in error count 1,latent period,and error count 2.Significant shortening of mean latency was observed in the rats of Al（mal）3 groups compared with that of the rats in saline group and in maltolate group（P0.05）,while error count 1 and 2 were increased significantly in Al（mal）3 groups,with significant differences in all Al（mal）3 groups except error count 2 in 0.27 mg/kg Al（mal）3 groups（P0.05）.Aluminum content in cerebral cortex in 0.27,0.54,and 1.08 mg/kg Al（mal）3 treated groups was 43.16±1.54,58.38±3.85,and 89.20±14.45 ng/g,respectively,which were increased significantly compared with those of saline group and maltolate group（P0.05）. Conclusion Subchronic exposure to Al（mal）3 can damage the rat＇s nervous system.