两种梅毒螺旋体血清学检测方法的临床应用评价

Clinical evaluation of two serological methods for detecting Treponema pallidum

目的探讨TRUST和TPPA联合检测对梅毒的监控和诊治价值,为临床诊治梅毒提供实验依据。方法采用TRUST法和TPPA法测定2 083例血清标本(其中910例梅毒患者血清)。结果对910例梅毒患者血清检测,TRUST法检出773例阳性,敏感性为84.9%,特异性为96.1%。TPPA法检出899例阳性,敏感性为98.7%,差异有统计学意义(P<0.05);TPPA法具有很高的特异性和灵敏度。结论 TRUST和TPPA联合检测为临床诊治梅毒提供更有价值的实验依据,更有利于梅毒的监控和诊治。

Objective To explore the diagnostic value of TRUST and TPPA in diagnosis of human syphilis and provide experimental theory for the clinical purpose.Methods TRUST and TPPA were used to detect 2 083 serum specimens（including 910 syphilis serum specimens）.Results There were 773 positives were detected by TRUSTout of 910 syphilis serum specimens with a positive rates of 84.9 % and a specificity of 96.1%;while there 889 specimens were positive detected by TPPA with the sensitivity and specificity of 98.7 %,there were significant differences in the outcomes between TPPA reagent and TRUST reagent.The specificities and the sensitivities of TPPA were 99.6% and 98.7%;higher than that of TRUST（84.9% and 96.1%）.Conclusion TPPA in combination with TRUST is valuable for detecting and diagnosing of human syphilis.