2-甲氧基雌二醇大鼠血浆蛋白结合率的测定

Determination of rat plasma protein binding rate of 2-methoxyestradiol

目的建立测定2-甲氧基雌二醇大鼠血浆蛋白结合率的方法。方法采用平衡透析法对2-甲氧基雌二醇大鼠血浆蛋白结合率进行HPLC测定。结果高、中、低3个浓度(2、4、8 mg.L-1)的2-甲氧基雌二醇血浆蛋白结合率分别为(85.2±3.4)%、(82.1±5.3)%、(83.0±5.2)%。结论该方法灵敏度高,重现性好,操作简单,能满足生物样品分析要求。2-甲氧基雌二醇大鼠血浆蛋白结合率不具有浓度依赖性,使其临床用药具备较好的安全性。

AIM To develop a HPLC method for the determination of the plasma protein binding rate of 2-methoxyestradiol （2-ME） in rat. METHODS The equilibrium dialysis was carried out to determine the protein binding rate of 2-ME in rat plasma. RESULTS middle and low concentrations （2, 4, 8 mg·L-1） were respectively. CONCLUSION The method is sensitive, The plasma protein binding rates of 2-ME at high, （85.2 ± 3.4） %, （82.1 ± 5.3） %, （83.0 ±5.2） %, accurate, simple and reproducible, and meets the requirement of biological sample analysis. The plasma protein binding rate of 2-ME shows no concentration dependence, which provides better security for clinical medication.