来氟米特对胶原性关节炎大鼠CD4^+CD25^+调节性T细胞的影响

Effect of leflunomide on CD4+CD25+ regulatory T cells in collagen-induced arthritisrats

目的:探讨来氟米特对胶原性关节炎中免疫抑制性CD4+CD25+调节性T细胞的作用。方法:II型胶原诱导Wistar大鼠,建立胶原性关节炎模型;检测关节炎大鼠多发性关节炎评分,每周2次;流式细胞仪检测脾淋巴细胞中CD4+CD25+调节性T细胞的比例;RT-PCR检测脾淋巴细胞中Foxp3mRNA的表达。结果:与模型组比较,来氟米特(2mg/kg,灌胃给药15d)明显降低CIA大鼠多发性关节炎评分。与关节炎模型组比较,药物干预组CD4+CD25+调节性T细胞的比例升高,转录因子Foxp3mRNA的表达增高。结论:来氟米特具有抑制关节炎大鼠关节肿胀的作用,该作用可能和促进CD4+CD25+Treg产生和增加Foxp3的表达有关。

Objective：To investigate the effect of leflunomide on the immunosuppressive CD4^＋CD25^＋ regulatory T cells（CD4^＋CD25^＋ Tregs）in collagen-induced arthritis（CIA）.Methods：CIA was induced by collagen type II in Wistar rats.Polyarthritis indexes were checked twice a week.Immunofluorescence flow cytometry and RT-PCR were used to determine the proportion of CD4^＋CD25^＋ Tregs and the expression of Foxp3 mRNA in the splenocytes of CIA rats,respectively.Results：Leflunomide（2 mg/kg,ig×15 days,day 10-24）could significantly decrease the polyarthritis indexes in CIA rats.Compared with model CIA rats,administration of leflunomide could significantly increase the number of CD4^＋CD25^＋ Tregs and the expression of Foxp3 mRNA in spleen lymphocytes of CIA rats.Conclusions：The results demonstrated that the inhibition of arthritis by leflunomide was related to changes in CD4^＋CD25^＋ Tregs.