检测流感病毒的商业PCR试剂盒比对验证

Validation of Commercial RT-PCR and Real-time RT-PCR Kits for Detection of Influenza Virus

利用8种不同流感病毒株及其核酸,结合自然感染组织样品与人工混合样,验证比较了8种商业PCR试剂盒的质量参数。结果表明,3号荧光PCR试剂盒漏检1株外,其他试剂盒均可以检测8种毒株,对其他类似病毒如猪繁殖与呼吸综合征病毒、猪伪狂犬病毒、猪传染性胃肠炎病毒、猪细小病毒和猪环状病毒核酸等无交叉反应,最高可检测到10-7稀释的目标核酸,重复性与再现性均为理想,在有效期内检测结果没有发生变化。

Five commercial real-time RT-PCR kits and three RT-PCR kits were assessed with eight influenza viruses.The different criteria assessed were sensitivity,specificity,reproducibility using different samples.These samples were inactivated strains or organs naturally infected or Spiked biological samples.The results proved these kits to be sensitive with high specificity and reproducibility.