摘要
从接种核盘菌(Sclerotinia sclerotiorum)后的甘蓝型油菜品种中双9号cDNA芯片中筛选出一个新的抗性相关基因BnERF50。序列分析表明该基因产物BnERF50蛋白含有一个典型的ERF(乙烯相应转录因子)保守结构域,属于ERF家族成员。接种核盘菌24h后,该基因的表达量被诱导显著上调。在它的超表达转基因拟南芥中,病原相关蛋白(PR)防御素基因PDF1.2和几丁质酶基因ChiB的表达量升高,对腐生营养型真菌核盘菌的抗性显著增强。
A novel BnERF50 gene was identified from transcription cDNA chip of Zhongshuang 9(Brassica napus L.) against Sclerotinia sclerotiorum infection.BLAST result showed BnERF50 protein has a typical and conserved ERF(ethylene response factor) domain which belongs to ERF family.The expression of BnERF50 was up-regulated significantly at 24h after S.sclerotiorum inoculation on B.napus.Over-expression of BnERF50 in transgenic Arabidopsis thaliana activated the expressions of pathogenesis-related(PR) genes PDF1.2 and ChiB,and it also significantly enhanced the plant resistance to the necrotrophic fungi S.sclerotiorum.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2011年第5期427-432,共6页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金项目(30671119)
国家863计划项目(2006AA10A113)