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甘蓝型油菜BnERF50的超表达增强了转基因拟南芥对核盘菌的抗性 被引量：2

Over-expression of BnERF50 from Brassica napus enhanced transgenic Arabidopsis resistance to Sclerotinia sclerotiorum
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摘要 从接种核盘菌(Sclerotinia sclerotiorum)后的甘蓝型油菜品种中双9号cDNA芯片中筛选出一个新的抗性相关基因BnERF50。序列分析表明该基因产物BnERF50蛋白含有一个典型的ERF(乙烯相应转录因子)保守结构域,属于ERF家族成员。接种核盘菌24h后,该基因的表达量被诱导显著上调。在它的超表达转基因拟南芥中,病原相关蛋白(PR)防御素基因PDF1.2和几丁质酶基因ChiB的表达量升高,对腐生营养型真菌核盘菌的抗性显著增强。 A novel BnERF50 gene was identified from transcription cDNA chip of Zhongshuang 9（Brassica napus L.） against Sclerotinia sclerotiorum infection.BLAST result showed BnERF50 protein has a typical and conserved ERF（ethylene response factor） domain which belongs to ERF family.The expression of BnERF50 was up-regulated significantly at 24h after S.sclerotiorum inoculation on B.napus.Over-expression of BnERF50 in transgenic Arabidopsis thaliana activated the expressions of pathogenesis-related（PR） genes PDF1.2 and ChiB,and it also significantly enhanced the plant resistance to the necrotrophic fungi S.sclerotiorum.
作者 汪承刚 李丹 许李明 董彩华 黄军艳 方菊萍 刘胜毅
机构地区 中国农业科学院油料作物研究所
出处 《中国油料作物学报》 CAS CSCD 北大核心 2011年第5期427-432,共6页 Chinese Journal of Oil Crop Sciences
基金 国家自然科学基金项目(30671119) 国家863计划项目(2006AA10A113)
关键词 乙烯响应转录因子 BnERF50 病原相关蛋白基因 核盘菌 Ethylene response factor BnERF50 gene Pathogenesis-related gene Sclerotinia sclerotiorum
分类号 S565.403 [农业科学—作物学] Q786 [生物学—分子生物学]
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参考文献18

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二级参考文献32

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共引文献8

同被引文献29

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二级引证文献28

中国油料作物学报
2011年 第5期

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