兔颈动脉粥样硬化斑块内MMP-2与新生血管关系的研究

The relationship between MMP-2 and the neovascularization in rabbit carotid atherosclerotic plaque

目的探讨兔颈动脉粥样硬化模型斑块内基质金属蛋白酶-2(matrix metaloproteinase-2,MMP-2)与新生血管的关系。方法将40只健康雄性新西兰大白兔随机分为4组,A组予普通饲料喂养28 d、B组予高脂饮食28 d、C组予硅橡胶圈干预联合高脂饮食14 d、D组予硅橡胶圈干预联合高脂饮食28 d。利用超声造影检测C组、D组兔颈动脉粥样硬化模型斑块内新生血管情况,应用免疫组化检测斑块内MMP-2、血小板内皮细胞黏附分子-1(cluster of differentiation 31,CD31)的表达,进行两者间相关性分析。结果超声造影检测到C组、D组模型兔颈动脉斑块内有新生血管形成,两组间差异有统计学意义(P<0.05)。免疫组化结果显示斑块内有阳性表达的MMP-2、CD31,两者之间呈正相关(P<0.01)。结论 MMP-2在兔颈动脉粥样硬化斑块内新生血管的生成中发挥一定作用。

To detect the relationship between matrix metaloproteinase-2（MMP-2） and the neova-scularization in rabbit carotid atherosclerotic model plaque,forty of the male New Zealand white rabbits were randomly divided into 4 groups： group A（the blank control group）,group B（high-cholesterol diet group）,group C（silicone collar intervention and high-cholesterol diet for 14 days group）,group D（silicone collar intervention and high-cholesterol diet for 28 days group）.We used contrast-enhanced ultrasound（CEUS） to detect the neovascularization in the carotid atherosclerosis plaque.The expressions of MMP-2 and cluster of differentiation 31（CD31） were assessed by immunohistochemical staining,and we analyzed the correlations between them.CEUS showed that the neovascularization in the carotid atherosclerotic model plaques was statistical difference between the C and D group（P 0.05）;immunohistochemistry showed there were positive MMP-2 and CD31 expressions in the plaques,and the correlative analysis showed the expressions were positive correlated（P 0.01）.We can conclude that MMP-2 play some roles in the neovascularization within the rabbit carotid atherosclerotic plaques.