金银花提取物对单纯疱疹病毒性角膜炎的作用

Effect of Flos Lonicerae Extracts on Herpes Simplex Keratitis

目的考察金银花提取物对单纯性疱疹病毒性角膜炎的疗效。方法建立单纯性疱疹病毒Ⅰ型(herpessimplex virus-Ⅰ,HSV-Ⅰ)感染Vero细胞的体外模型,以细胞病变效应、治疗指数为观察指标,了解不同浓度金银花提取物体外抗病毒效果;采用HSV-Ⅰ感染家兔角膜建立体内病毒感染模型,分别用金银花提取物、阿昔洛韦、0.9%氯化钠溶液治疗15 d,每天经荧光素钠染色角膜后在裂隙灯下观察药物对病毒性角膜炎模型的治疗作用。结果体外实验表明,金银花提取物对感染HSV-Ⅰ病毒的Vero细胞具有明显的抗病毒作用,最大无毒浓度为384 mg.L-1,治疗指数为26.56;体内实验结果显示,与模型组相比,金银花提取物和阿昔洛韦均能有效治疗单纯疱疹病毒性角膜炎,减轻角膜病变程度,缩短平均治愈时间,金银花提取物疗效与阿昔洛韦相似。结论金银花提取物对单纯疱疹病毒性角膜炎有显著的疗效。

Objective To study therapeutic efficacy of Flos Lonicerae extracts on herpes simplex keratitis. Methods A model of Vero cells infected with herpes simplex virus-Ⅰ（HSV-Ⅰ） was established.The in vitro anti-virus effect of different doses of Flos Lonicerae extracts was evaluated by using cytopathic effect（CPE） and therapeutic index（TI）.In vivo model of rabbit corner keratitis induced with herpes simplex（HSK） were established.Rabbits were treated with Flos Lonicerae extracts,Acyclovir（ACV） and normal saline for 15 days,respectively.The rabbit cornea was checked after dyeing with flourescein-natrium under slitlamp. Results It was showed that the Flos Lonicerae extracts possessed distinct anti-HSV-Ⅰ effect on Vero cells,TC0 of which was 384 mg·L-1and TI was 26.56.Compared with the model group in vivo,both Flos Lonicerae extracts and ACV significantly reduced severity of HSV-Ⅰdendritic keratitis,shortened healing time of HSK.There was no statistical significance between Flos Lonicerae extracts and ACV（P0.05）. Conclusion Flos Lonicerae extracts are effective against HSK.