摘要
目的以黄酮含量为评价指标,优选睡莲花超声提取最佳工艺。方法单因素实验考察乙醇浓度、料液比、超声提取时间、超声提取次数、超声功率对药材黄酮提取效果的影响,并通过正交实验确定最佳提取工艺。结果乙醇浓度、料液比、提取次数和提取时间对黄酮的提取率有显著影响。超声提取维药睡莲花中黄酮类化合物的最佳工艺条件为:乙醇浓度80%,料液比1∶16,超声提取5次,提取时间30 min。结论所优选的提取工艺稳定,重复性良好。
Objective To optimize the ultrasonic extraction for total flavonoids from Nymphaea candida Presl. Methods The optimum process was determined with the single factor experiment and orthogonal test by evaluating effect of ethanol concentration,ratio of materia/extraction solution,extraction course,times and amount of total flavonoids extracted. Results The remarkable factors affecting total flavonoids extraction from Nymphaea candida Presl.included ethanol concentration,ratio of materia/extraction solution,extraction times and extraction duration.The optimum extraction for total flavonoids from Nymphaea candida Presl.was using 80% ethanol,keeping the ratio of materia/extraction solution as 1：16,making extraction for 5 times and 30 minutes in each time. Conclusion The optimum process is stable and reproducible.
出处
《医药导报》
CAS
2011年第11期1487-1489,共3页
Herald of Medicine
关键词
睡莲花
总黄酮
超声提取
正交实验
Nymphaea candida Presl.
Total flavonoids
Ultrasonic extraction
Orthogonal test
