多孔陶粒固定化微生物效果及扫描电镜观察

Effect of porous ceramic on the immobilized microorganisms and scanning electron microscopy

采用0.2 mol/L和1.0 mol/L的HCl以及0.2 mol/L和1.0 mol/L的NaOH、铁盐和铝盐,分别对陶粒进行改性,将未处理陶粒和改性陶粒分别在30℃、60 r/min条件下吸附恶臭假单胞菌,通过测定不同时间上清液OD600值来比较它们的吸附效果,并用扫描电镜对吸附效果进行观察.结果表明:低浓度酸处理陶粒吸附菌效果比碱处理和高浓度酸处理好,其上清液的相对OD600值差最大.未处理陶粒、低浓度酸处理陶粒、铁膜和铝膜陶粒对菌都有较好的吸附能力,吸附率随时间延长而增大,3.5 h后它们的吸附率分别为27.70%、34.84%、36.83%和31.69%,其中铁膜陶粒吸附效果最好,扫描电镜观察与吸附实验结果相一致.把微生物吸附在陶粒上处理废水,能结合它们处理废水的优势,且可以回收陶粒,将为研究改性陶粒固定化微生物在废水处理中的作用和效果提供较直观的依据.

By acid treatment,aluminum-iron salt processing and handling of modified ceramic,under the conditions of 30 ℃ and 60 r/min,using untreated ceramic and three kinds of modified ceramic to adsorb the Pseudomonas putida and compare their adsorption.Then using a scanning electron microscope to observe the adsorption effect.The results showed that：four kinds of different treated ceramic had good adsorption capacity,and their adsorption rate increased with time,after 3.5 hours their absorption rates were 27.70%,34.84%,36.83% and 31.69%,respectively,which iron film ceramic absorption was the best.The observation of scanning electron microscopy was consistent with the adsorption effects.Adsorbing the microbes on the ceramic in wastewater treatment,can combine their strengths,and ceramic can be recycled.It can provide an intuitive basis for studying the role and effect of the modified ceramic immobilized microorganisms in wastewater treatment.