正交实验法优选感冒清热颗粒的制备工艺

Study on preparation technique for Ganmaoqingre Granulse by orthogonal experimental design

目的研究感冒清热颗粒的制备工艺。方法采取水蒸气蒸馏法提取挥发油,正交实验设计确定挥发油β-环糊精(β-CD)包合的最佳工艺,以葛根素转移率为指标,优选提取工艺。结果挥发油提取条件为:加10倍量水提取6h;环糊精最佳包合工艺为:油与β-CD比例为1∶6,恒温40℃搅拌包合1h;煎煮最佳工艺为:加8倍水,煎煮2次,每次1.5h。结论感冒清热颗粒的制备工艺稳定、可行。

Objective To optimize the preparation procedure of Ganmaoqingre Granules.Methods The volatile oil was extracted by steam-distillation method and included with β-cyclodextrin（β-CD）.Orthogonal experiment was introduced to optimize the conditions of decoction and volatile oil inclusion,with kakonein transferring ratio as a marker to evaluate the decoction conditions.Results The optimum extraction conditions were as follows：the crude drug which contains volatile oil was extracted with 10 times amount of water for 6 hours to collect the volatile oil.The optimal inclusion conditions selected by the orthogonal design were as follows：the ratio of volatile oil to β-CD was 1∶6,the inclusion time was 1 h and the temperature of inclusion was 40 ℃.The optimized conditions for decoction were 2 times（1.5 h/time） by water with 8 times amount of water.Conclusion This optimized preparation procedure for Ganmaoqingre Granules is stable and feasible.