HPLC法同时测定注射用头孢他啶和精氨酸的含量

Simultaneous determination of Ceftazidime and Arginine by HPLC

目的建立同时测定注射用头孢他啶（含精氨酸）中头孢他啶和精氨酸的含量的高效液相色普法（HPLC）。方法采用Thermo pHenyl-2柱,乙腈：醋酸盐缓冲液（5∶95）为流动相,检测波长206nm。结果头孢他啶、精氨酸线性范围分别为0.09196～0.9196mg/ml和0.04868～0.4868mg/ml,回收率分别为99.97%~100.59%和99.80%～101.59%,相对标准偏差（RSD）分别为0.04%~0.74%、0.02%～1.02%（n=9）。结论该法专属性好、快速、准确。

Objective To develop a reversed-phase high performance liquid chromatographic （RP-HPLC） method for the simultaneous determination of ceftazidime and arginine in injectable powder. Methods A HPLC method was established applying Thermo Hypersil Phenyl-2 Column （250 mm×4.6 mm 5μm）, with a mobile phase of acetate buffer and acetonitrile （95：5）. The detection wavelength is 206 nm with a flow rate at 1.0 ml/min and column temperature at 30℃. Results The linear ranges of ceftazidime and arginine were 0.091 96~0.919 6 mg/ml and 0.048 68~0.486 8 mg/ml, respectively. The average recoveries were 99.97%~100.59% for ceftazidime, and 99.80%~101.59% for arginine. The RSD values of inter-day and intra-day assays were 0.04%~0.74% and 0.02%~1.02% for ceftazidime and arginine, respectively. Conclusion The method developed is convenient, rapid, accurate and precise.