川芎嗪配伍黄芪多糖对血管内皮细胞的保护作用

The protective effects of Tetramethylpyrazine(TMP) combined with Astragalus Polysaccharides(APS) on vascular endothelial cells(VECs)

目的:探讨川芎嗪(TMP)、黄芪多糖(APS)及其配伍对血管内皮细胞(VECs)的保护作用。方法:用浓度为10%高血压病患者血清作用于脐静脉内皮细胞株(ECV-304)24h进行造模,实验分为模型组、对照组、TMP组、APS组、TMP与APS配伍组。其中药物各组是将患者血清与药物共同培育细胞。以四甲基偶氮唑盐(MTT)法检测细胞活性,硝酸还原酶法检测一氧化氮(NO)浓度,非平衡法测定内皮素(ET),激光扫描共聚焦显微镜检测细胞内游离钙浓度。结果:各药物组浓度依赖性增强细胞活性、增加NO释放、而抑制细胞ET释放、增加胞内游离钙浓度,且这两个中药单体配伍后比单独使用作用更显著。结论:TMP、APS及其配伍对VECs具有明显保护作用,且两者可能存在一定的协调作用。

Objective： To study the protective effects of tetramethylpyrazine（TMP）,astragalus polysaccharides（APS） and their combination on vascular endothelial cells（VECs）.Methods： To establish the cell model,We selected that the serum concentrations was 10% and the time was 24h,at the same time ECV-304 were processed by TMP,APS and their different combinations.Experiments were divided into 5 groups： the model group,the control group,the TMP groups,the APS groups and the monomer combination groups.the activeness of cultivated ECV-304 were assayed by MTT chromatometry,the nitric oxide（NO） excreted by the cells was assayed by nitric acid deoxidizing enzyme method,and the endothelin（ET） was assayed by non-balance method,the cells＇ intracellular free calcium（i） was assayed by laser scanning confocal microscope（LSCM）.Results： Medicine groups could strengthen cells＇ activeness,increased the NO level,decreased ET level and cells＇ intracellular free calcium significantly compared with the model group.Conclusion： We considered that TMP and ASP has the distinct protective effects on VECs.We also found that TMP combined with APS was superior to a single medicine on the protective effects on VECs.