泰山白花丹参提取物对人脐血内皮祖细胞增殖、黏附及分泌NO的影响

Effects of Taishan Salvia miltiorrhiza Bunge.f.alba extract on proliferation,adhesive activity and nitric oxide secretion of umbilical cord blood endothelial progenitor cells

目的:观察单味中药泰山白花丹参提取物(SBE)对人脐血内皮祖细胞(EPCs)增殖、黏附及分泌一氧化氮(NO)的影响。方法:采用密度梯度离心法从脐血获得单个核细胞,将其接种在人纤维连接蛋白包被的培养板上,培养7d后收集贴壁细胞,加入不同浓度SBE继续培养24h;分别采用MTT比色法、黏附能力测定实验和硝酸还原酶法测定EPCs的增殖、黏附和分泌NO能力。结果:与对照组比较,SBE组EPCs增殖、黏附能力提高,培养上清中NO浓度增加(P<0.05),其作用在一定范围内随SBE浓度增加而增强。结论:白花丹参显著改善EPCs功能,这可能是其内皮保护的新机制。

Objective： To investigate the effects of single Chinese traditional medicine of Taishan Salvia miltiorrhiza Bunge.f.alba extract（SBE） on proliferation,adhesive activit of endothelial progenitor cells（EPCs） and its nitric oxide（NO） secretion.Methods： Total mononuclear cells were isolated from human umbilical cord blood by ficoll density gradient centrifugation and inoculated on the human fibro-ligandin encrusting plate.After 7 days of in vitro culture,adherent cells were collected and incubated with SBE for 24h.The proliferation,adhesive activity and NO secretion of EPCs were assayed using MTT,adhesion determination and nitrate reductase method,respectively.Results： EPCs incubated with SBE showed the capacities higher than those of control in proliferation,adhesion and with a higher NO concentration in the culture supernatant.Conclusion： Salvia miltiorrhiza Bunge.f.alba can improve the function of EPCs,which might be a novel mechanism of its effects in improving vascular endothelial function.