七叶皂苷对人肝癌HepG-2细胞增殖与凋亡的实验研究

Experimental Research of Qiyezaogan's Influence to live cancer hepg-2

目的：观察研究七叶皂苷对人肝癌HepG-2细胞的增殖周期及凋亡的影响,探讨七叶皂苷抗肿瘤作用的机制。方法：噻唑蓝（MTT）法检测七叶皂苷对人肝癌HepG-2细胞生长的抑制作用,流式细胞仪分析七叶皂苷对人肝癌HepG-2细胞周期及凋亡的影响。结果：七叶皂苷对人肝癌HepG-2细胞的增殖有明显的抑制作用,并随着作用浓度的增大,抑制作用逐渐增强。流式细胞仪检测可见亚二倍体峰,提示七叶皂苷有诱导人肝癌HepG-2细胞凋亡作用。七叶皂苷可通过诱导人肝癌HepG-2细胞凋亡并将细胞阻滞在G0~G1期,使细胞不能进入S期进行DNA合成,最终使瘤细胞的体外增殖受到抑制。结论：七叶皂苷抑制人肝癌HepG-2细胞的增殖,使细胞周期阻滞和诱导细胞凋亡,七叶皂苷可能在其抗肝癌作用中具有重要意义。

Objective： Observe and study the effect of aescine into the proliferation cycle and apoptosis of liver cancer cell HepG-2;Discuss the mechanism of aescine against tumor.Methods： Test the inhibition of aescine against liver cancer cell HepG-2 by MTT assay and analyze the effect of aescine into the life cycle and apoptosis of liver cancer cell HepG-2 by flow cytometer.Results：Aescine inhibits the proliferation of liver cancer cell HepG-2 obviously,and with the higher concentration,the stronger effects will be accomplished.Summit of hypodiploid can be seen by flow cytometer,which infers that aescine can induce apoptosis of liver cancer cell HepG-2.By inducing the apoptosis of liver cancer cell HepG-2 and blocking them within phase of G0-G1,aescine disables the DNA synthesis in the S phase and gets a final inhibition of the tumor cells＇ proliferation in vitro.Conclusion： Aescine inhibits the proliferation of liver cancer cell HepG-2,blocks the life cycle and induces the apoptosis.Aescine has certain significance in the treatment of liver cancer.