一种叶绿素实时检测的新方法

A New Method of Real-time Detecting the Chlorophyll

介绍了一种基于MINIPAM荧光仪调制脉冲式荧光检测技术的LED辐照叶绿素荧光参数的实时检测方法。该方法利用可编程电源为LED列阵提供恒流输出驱动,LED列阵作为激发叶绿素产生荧光的光化光,利用MINIPAM荧光仪对植物光系统II的稳态荧光Fs、光适应下最大荧光Fm’进行检测,并结合环境测控系统的测量,综合分析环境因素对实际量子效率、实际电子传递速率等参数的影响。MINIPAM荧光仪通过与上位机通信,可以进行实时在线监控。

A fluorescent real-time detection technology parameters of the chlorophyll radiation LED method using with fluorescent MINIPAM based on impulse,modulation,is introduced.The using of programming power to provide a constant flow of the list of output,LED by a matrix as chlorophyll are fluorescent light.The using of apparatus in plant MINIPAM fluorescent light system II of the steady state Fs,a fluorescent light to the next largest fluorescent Fm＇,a combination of the environment,and tracking control system of measurement and comprehensive analysis of environmental factors to the real quantum efficiency,actual electronic transmission rate for parameters.MINIPAM fluorescent apparatus with the upper machine,and can be real time monitoring.