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慢病毒载体及应用研究进展 被引量：7

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摘要 慢病毒（Lentiviral）又叫逆转录病毒,属于逆转录病毒科（Retrovidae）,是一种RNA病毒。由于这种病毒中含有逆转录酶,故名逆转录病毒。慢病毒在宿主细胞内,能以RNA为模板,在自身反转录酶的作用下合成cDNA,再以cDNA为模板合成双链DNA,经环化后通过病毒整合酶作用整合在宿主细胞的染色体上并长期表达。慢病毒载体（Lentiviral Vector,LV）是由慢病毒以其基因组为基础去除部分基因,代之以所需的目的基因和标记物构建而成,与其它载体比较,慢病毒载体具有携带基因片段容量大、转染效率较高、
作者 张薇 赵志英 张坤
机构地区 包头医学院第一附属医院眼科 包头医学院
出处 《包头医学院学报》 CAS 2011年第5期107-109,共3页 Journal of Baotou Medical College
关键词 慢病毒载体 逆转录病毒科 RNA病毒 应用 CDNA 宿主细胞 双链DNA 逆转录酶
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献17

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二级参考文献17

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共引文献33

同被引文献56

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引证文献7

二级引证文献16

包头医学院学报
2011年 第5期

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