UV-Vis法测定猕猴桃根提取物多糖的含量

Determination of polysaccharide in Kiwi fruit root by UV-Vis

目的建立从猕猴桃根中提取多糖，并测定多糖含量的方法。方法以UV-Vis法为检测手段．应用苯酚．硫酸法测定称猴桃根提取物多糖的含量。结果在10．02-60．12μg·mL-1范围内呈良好的线性关系，回归方程为：A=0．0159C＋0．028（r=0．9997），猕猴桃根多糖平均回收率为100．41％，RSD为1．10％．样品含量为56．74％，RSD为1．21％。结论本方法可靠、简便、准确，可用于称猴桃根中多糖的含量测定。

OBJECTIVE To establish a method for extracting the polysaccharide from Kiwi fruit root and determinate the content of polysaccharide. METHODS Determination the polysaccharide in kiwifruit root extracting by UV-vis and phenol-sulfuric. RESULTS In 10.02--60.12μg·mL-1 range is good linear relationship, the regression equation is： A = 0. 0159C ＋ 0. 028 （r = 0. 9997）, The average recovery of Kiwi fruit root polysaccharide is 100.41%, RSD is 1.10 %, and the sample content is 56.74 %, RSD is 1.21%. CONCLUSION The method is reliable, simple, accurate and can be applied to determine the polysaccharide in Kiwi fruit root.