HPLC法测定美扑伪麻片中对乙酰氨基酚含量

Determination of Paracetamol in Compound Dextromethorphan Hydrobromide tablets by HPLC

目的建立HPLC法测定美扑伪麻片中对乙酰氨基酚的含量。方法0．05mol·L-1。磷酸二氢钾-甲醇-三乙胺（80：20：0．02％），用磷酸调节pH3．5作为流动相，流速1．0mL·min－1．上样量20uL，检测波长243nm。结果对乙酰氨基酚在9．35－95．7μg·mL－1内，峰面积与浓度呈良好线性关系（r=1），回收率100．12％，RSD=0．64％（n=9）。结论本法能简单、准确、可靠地测定美扑伪麻片中对乙酰氨基酚的含量。

OBJECTIVE To establish a HPLC method for determination of paracetamol in Compound Dextromethorphan Hydrobromide tablets. METHODS The kromasil 100-C18 was used with the mobile phase of 0.05mol· L- l potassium primary phosphate-MeOH-triethylamine （80 ： 20 ： 0.02 % ） . Adjust the pH to 3.5 by phosphoric acid. The flow rate was 1.0mL· min-land the detection wavelength was 243nm. RESULTS The precision and stability were fine. The linear range within 9.35 -- 95.7μg·mL- 1, r = 1. The average recoveries were 100.12 %, RSD was 0.64 % （n = 9）. CONCLUSION The method was simple, accurate and reproducible, It can be used for the quality control of Compound Dextromethorphan Hydrobromide tablets.