二苯乙烯苷对老龄大鼠脑缺血再灌注后HIF-1α及EPO表达的影响

Effects of tetrahydroxystilbene glucoside on hypoxia inducible factor-1 alpha and erythropoietin in old rat after cerebral ischemia reperfusion

目的探讨二苯乙烯苷(TSG)对老龄大鼠脑缺血再灌损伤后的神经保护作用。方法雄性SD老龄大鼠72只,随机分为3组:即假手术组、模型组及TSG干预组,每组24只。其中模型组及TSG组分别灌胃生理盐水及TSG,6d后应用线栓法制备大鼠大脑中动脉缺血再灌注损伤模型,于术后6h、24h、48h及7d 4个时间点观察动物神经行为学变化并评分,免疫组化法检测HIF-1α和EPO蛋白的表达。结果神经功能评分显示模型组及TSG干预组各时间点均有明显的神经功能缺损症状,除6h时间点外,其余各时间点TSG干预组大鼠神经功能评分显著低于模型组(P<0.05);与模型组比较,TSG干预组各时间点HIF-1α及EPO蛋白表达均显著升高(P<0.01)。HIF-1α与EPO蛋白表达增加呈正相关。结论 TSG可能通过增加老龄大鼠脑缺血再灌注损伤缺血周边区HIF-1α及EPO蛋白的表达,起到脑保护作用。

Objective To investigate the effects of tetrahydroxystilbene glucoside（TSG） on neurological deficits,the expressions of hypoxia inducible factor-1 alpha（HIF-1α） and erythropoietin（EPO） in old rats after cerebral ischemia-reperfusion.Methods 72 Sprague-Dawley male old rats were divided into three groups（n=24）：control group,ischemia-reperfusion（I/R） model group and TSG（60mg/kg） group.After 6ds＇administration of TSG or natural saline（model group）,reversible middle cerebral artery occlusion（MCAO） model was established by intralu-minal suture technique.Rats in control group were operated while middle cerebral arteries were not blocked.At 6h,24h,48h and 7d after reperfusion,behavior test was used to evaluate the neurological deficiency of rats in each group.The expressions of HIF-1α and EPO in the cortex were measured by immunohistochemical method.Results Ischemia-reperfusion model group and TSG group rats had neurological deficits.Compared with model group,TSG could decrease the grade of the rat neurological defects except at 6h after reperfusion and increase the protein expressions of HIF-1a and EPO after reperfusion.Conclusion TSG can improve the neurological function through increasing the expressions of HIF-1a and EPO of cerebral ischemia-reperfusion old rats.