骨髓间质干细胞联合脑源性神经营养因子用于大鼠脑出血的实验研究

The experiment of exogenous BDNF combined with bone mesenchymal stem cells after intracerebral hemorrhage on adult rats

目的观察骨髓间质干细胞(MSCs)联合脑源性神经营养因子(BDNF)治疗大鼠脑出血(ICH)的疗效。方法建立大鼠ICH模型,体外培养标记纯化的MSCs,经侧脑室植入脑部,同时局部注入BDNF。记录对照组、BDNF组、MSCs组和MSCs+BDNF组7d、14d、21d大鼠神经功能改善程度;免疫组化法检测MSCs脑内迁移及分化;电子显微镜观察神经凋亡细胞。结果 MSCs组大鼠运动功能有明显改善,MSCs+BDNF组对ICH损伤的修复作用最明显。MSCs+BDNF组各时间点MSCs阳性细胞数及NEUN、GFAP、CNP免疫阳性细胞数均高于MSCs组(P<0.01),且MSCs+BDNF组神经细胞凋亡程度最轻。结论 MSCs脑部移植可促进大鼠ICH损伤部位结构和功能修复,BDNF对其修复具有协同作用。

Objective To observe the treatment effect of bone marrow mesenchymal stem cells（MSCs） combined brain-derived neurotrophic factor（BDNF） on the adult rats with intracerebral hemorrhage（ICH）.Methods We built rat ICH models,cultured and mark the purified MSCs in vitro,and implanted them into the brain through the left ventricle while using BDNF locally.Then we record the recovery of rats neurological function of control group,BDNF group,MSCs group and MSCs＋BDNF group after 7d,14d,21d.Immunohistochemistry methods was used to detect MSCs migration and differentiation in the brain.The level of neuronal apoptosis was observed with electron microscopy.Results Motor function of MSCs group improved significantly,ICH injury of MSCs＋BDNF group got most obvious repairment.BDNF＋MSCs group had a higher level of MSCs,NEUN,GFAP and CNP immunoreactive cells than MSCs group（P0.01） at each time point.MSCs＋BDNF group had the least number of inhibited apoptosis among all groups.Conclusion MSCs transplantation in rat brain improves repairing of the structure and the function of damaged parts due to ICH,and BDNF has a synergistic effect in this repairing process.