血培养常见病原菌分布及药敏分析

Distribution of common pathogens isolated from blood culture and drug susceptibility

目的了解医院门诊与住院患者血培养阳性标本检出的病原菌分布及耐药性,为临床合理应用抗菌药物提供参考。方法采用法国生物梅里埃公司的BacT/Alert240全自动血培养仪进行培养检测,阳性标本转种后用法国生物梅里埃公司API鉴定系统进行鉴定,然后用K-B法进行药敏测试。结果送检的2423份标本中,血培养阳性率10.73%,检出病原菌260株,其中革兰阳性球菌占53.46%,革兰阴性杆菌占42.69%,真菌占3.85%,检出率最高的病原菌为凝固酶阴性葡萄球菌、大肠埃希菌和肺炎克雷伯菌;主要革兰阳性菌对万古霉素、替考拉林,阿米卡星呈高度敏感,大肠埃希菌和肺炎克雷伯菌对亚胺培南/西司他丁、美罗培南100.00%敏感。结论血培养分离出的病原菌耐药率高,及时了解血培养结果,对临床有针对性的抗菌治疗,提高治愈率有重要意义。

OBJECTIVE To understand the distribution and drug resistance of common pathogens of positive blood culture from outpatients and inpatients, to provide clinical reference rational use of antimicrobial drugs. METHODS The French company bioM6rieux BacT/Alert240 automated blood culture system was used for culture tests. The species were identified by the French Merieux API identification system, and susceptibility testing was performed by using KB method. RESULTS Of 2423 cases of censorship specimens, 260 strains of pathogens were detected, blood culture positive rate was 10. 73%, Gram-positive cocci accounted for 53.46%, Gram-negative bacilli accounted for 42.69%, fungi accounted for 3.85 %, the highest detection rate of bacteria was coagulase-negative staphylococcus, Escherichia coli and Klebsiella pneumoniae ; Gram-positive bacteri＇a were highly sensitive to vancomycin, teicoplanin lalin, and amikacin. Escherichia coli and Klebsiella pneumoniae were 100.00 sensitive to imipenem/cilastatin and meropenem. CONCLUSION The drug resistance rates of the pathogens isolated from blood culture are high, to keep abreast of the results of clinical blood culture targeted antimicrobial therapy is of great significance to improve the cure rate.