HBeAg阴性乙型肝炎患者HBV-PreS1及HBV-DNA检测分析

Detection of serum HBV-PreS1 and HBV-DNA in HBeAg-negative hepatitis B patients with

目的分析HBeAg阴性的乙型肝炎患者血清HBV-PreS1及HBV-DNA检测的价值。方法收集114份HBeAg阴性的乙型肝炎患者血清标本,分别采用实时荧光定量PCR检测HBV-DNA、ELISA法检测HBV-PreS1抗原。结果 114例乙型肝炎患者血清中,HBV-DNA阳性69例,总检出率为60.5%,PreSl抗原阳性77例,总检出率为67.5%,HBV-DNA与PreSl抗原具有较好的相关性,HBeAg阴性的乙型肝炎患者血清HBV-DNA、HBV-PreS1阳性率差异无统计学意义;HBV-DNA复制拷贝数从<103～>107拷贝/ml,HBV-PreSl抗原检出率随HBV-DNA含量的增加逐渐上升。结论 HBeAg阴性的乙型肝炎患者血清中HBV-Pre-Sl抗原检测可以作为HBV-DNA检测的有效补充。

OBJECTIVE To analyze the clinical significance of serum HBV-PreS1 and HBV-DNA in patients with Hepatitis B whose HBeAg were negative. METHODS Serum specimens of 114 Hepatitis B patients with HBeAg negative were collected. HBV-DNA was detected using the real-time fluorescent quantitative PCR method and HBV-PreS1 was measured by ELISA. RESULTS Of all the 114 serum specimens, 69 cases of HBV-DNA and 77 cases of HBV-PreS1 was positive. The detection ratio was 60.5 % （69/114） and 67.5 % （77/114）, respectively. It showed that there were good relationship between HBV-PreS1 and HBV-DNA and there were no significant difference between them in patients with Hepatitis B whose HBeAg were negative （X2= 1.45, P〉 0.05）. When the HBV-DNA copies was between 10a and 107 , the detection ratio of HBV-PreS1 gradually increased with HBV-DNA. CONCLUSION Detection of HBV-PreS1 can serve as an effective alternative for the measurement of HBV-DNA in HBeAg-negative Hepatitis B patients.