复胃汤对大鼠胃癌前病变的防治作用研究

Fuwei decoction for prevention of gastric precancerous lesions in rats

目的 探讨中药复胃汤预防和治疗胃癌前病变的机制和效果.方法 对140只胃癌前病变模型大鼠进行中药（复胃汤）和西药（维酶素）预防和治疗给药,观察胃黏膜病理改变和Caspase-3的变化.结果 中药及西药预防组不典型增生发生率分别为50.0％（6/12）、42.86％（6/14）,明显低于造模组的90.0％（9/10）（H=16.40,H=16.86,均P＜0.05）.中药及西药治疗组不典型增生发生率为42.9％（6/14）和41.7 ％（5/12）,明显低于造模空白组的90.9 ％（10/11）（H=12.39,H=12.18,均P＜ 0.05）,且多为轻、中度不典型增生[45.45％（6/11）、36.36％（4/11）].造模组Caspase-3表达明显低于正常对照组（q=3.05,P＜ 0.01）,中药及西药预防组Caspase-3表达均增加,均明显高于造模组和正常对照组（q=5.45、q=7.54、q=8.51、q=10.60,均P＜ 0.01）.中药及西药治疗组Caspase-3表达亦明显高于造模空白组和正常组,差异均有统计学意义（q=15.66、q=18.53、q=15.66、q=18.53,均P＜ 0.01）.结论 复胃汤通过促进Caspase-3的表达,抑制了胃黏膜上皮细胞不典型增生。

Objective To investigate the treatment of Fuwei decoction for the prevention of gastric precancerous lesions and to elucidate the possible mechanism and medical effect underlying the activity.Methods A total of 140 rats with gastric precancerous lesions were utilized through traditional Chinese medicine （CM） and Western medicine prevention and treatment,in order to observe the various clinical changes of gastric mucosal pathology and Caspase-3.Results The results showed that the incidence of atypical hyperplasia treated by TCM group was 50.0% （6/12） and that by western medicine prevention group was 42.86% （6/14）,which was significantly lower than gastric mucosal rebuilding group [90.0% （9/10）]（H =16.40,H =16.86,p〈0.05）.Compared with gastric mucosal rebuilding gap group 90.9 %（10/11）（H =12.39,H =12.18,P 〈0.05）,the incidence of atypical hyperplasia （A＆H） underlying TCM group and western medicine prevention group were 42.9%（6/14）and 41.7% （5/12）,respectively,which much of them was further confirmed as mild and moderate A＆H [45.45% （6/11）,36.36 %（4/11）].Furthermore,also it was found that all the groups,turn out to express with obvious rate discrepancy underlying Caspase-3 as：（1） the rate of gastric mucosal rebuilding group was lower than regular comparison group （q =3.05,P 〈0.01）; （2） the rate of TCM group ＆ western medicine prevention group is much higher than gastric mucosal rebuilding group ＆ regular comparison group （q =5.45,q =7.54,q =8.51,q =10.60,P 〈0.01）; （3） the rate of TCM group ＆ western medicine treatment group is also rather higher than gastric mucosal rebuilding gap group ＆ regular comparison group （q =15.66,q =18.53,q =15.66,q =18.53,P 〈0.01）.Conclusion The findings of the current study illustrate that the Fuwei decoction has a protective ＆ preventive effect on gastric precancerous.which is against the atypical hyperplasia on gastric epithelial cells underlying Caspase-3