RhoA蛋白在胃癌组织中的表达及临床意义

Expression and significance of RhoA protein in gastric carcinoma tissues

目的:检测RhoA蛋白在不同临床分期胃癌组织中的表达,研究RhoA与胃癌发生和侵袭转移的关系,探讨其在胃癌发生、发展中的作用。方法:选取有完整临床资料的57例胃癌病例、23例非典型增生病例石蜡包埋组织,并27例非胃癌组织病例的石蜡包埋组织标本作为对照组,应用免疫组化法分别检测3组病例中RhoA蛋白的表达情况。结果:RhoA蛋白在胃癌组织中表达率为70.2%,在非典型增生组织中表达率为51.9%,在非胃癌组织中表达率为8.7%。胃癌、非典型增生和对照组3组病例间的两种蛋白表达两两比较差异都有显著的统计学差异(P<0.05)。RhoA蛋白表达与性别、年龄无关,和细胞分化、病理分期、淋巴结转移有关。结论:RhoA蛋白表达可能与胃癌的发生有关,参与了胃癌的浸润转移及淋巴转移,可作为胃癌侵袭、转移的重要生物学标志。

Objective：To determine the different expressions of RhoA protein in gastric carcinoma tissues in different clinical stages and study RhoA＇s correlation with the occurrence,invasion and metastasis of gastric carcinoma,and its role in the occurrence and develepment of gastric carcinoma. Methods;57 specimens of gastric carcinoma patients with complete clinical data,23 specimens of paraffin-sectioned tissues of normal epithelium and dysplasia,and 27 specimens of paraffin-sectioned tissues of normal gastric tissues（control group） were selected.The expressions of RhoA in patients of the three groups were detected by immunohistochemistry method.Results：The positive expression rates of RhoA in gastric carcinoma,normal gastric epithelial dysplasia and gastric tissues were 70.2%,51.9%and 8.7%respectively.There was significantly statistical differences between the three groups in RhoA expressions（P0.05）.The expression of RhoA had no relation with sex and age of patients,but it was correlated to cell differentiation,clinical stage,lymph node metastasis.Conclusions：The expression of RhoA may be correlated with the occurrence of carcinoma. It participates in the invasion and lymph node metastasis of gastric carcinoma,which might be an important biological marker for the invasion and lymph node metastasis of gastric carcinoma.