水稻细菌性条斑病菌拮抗细菌的筛选、评价与应用研究

Screening,Evaluation and Utilization of Antagonistic Bacteria against Xanthomonas oryzae pv.oryzicola

从江苏省南京、泰州、扬州和宿迁等地区水稻田采集水稻病叶、健叶、根围土等样品90份,分离纯化得到1173个细菌分离物,对其进行水稻细菌性条斑病菌Xanthomonas oryzae pv.oryzicola皿内抑制试验,共获得12株拮抗能力较强的细菌,其中4株拮抗细菌抑菌圈直径大于27mm。盆栽试验结果表明,12株拮抗能力较强的细菌中有4株对水稻细菌性条斑病菌防效大于50%,其中菌株Lx-11盆栽防效达62.5%。大面积示范试验结果表明菌株Lx-11田间防效达60.2%,显著高于化学药剂20%叶枯唑的防效(51.2%和45.8%)。形态特征、理化特性和分子鉴定分析确定菌株Lx-11为解淀粉芽孢杆菌Bacillus amyloliquefaciens。

In this paper, 1173 bacterial strains were isolated fromXanthomonas oryzae pv. oryzicola leaves infected, healthy leaves and rhizosphere of rice plants sampled in Nanjing, Taizhou, Yangzhou and Suqian of Jiangsu province. Among the isolated strains, 12 strains demonstrated high inhibition against X. oryzae pv. oryzicola. Diameter of inhibition zones of 4 strains were more than 27 mm. Control efficiencies of strain Lx-11 were 62.5% and 60.2% in pot and field trials, respectively, significantly higher than those of 20% bismerthlazol treatment （51.2% and 45.8%）. Based on morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis, the strain Lx-11 was identified to be Bacillus amyloliquefaciens.