虎眼万年青提取物体外清除自由基活性的研究

Study on Free Radical Scavenging Activity of Ornithogalum caudatum Ait Extracts in vitro

[目的]研究虎眼万年青不同体积分数乙醇提取物体外抗氧化作用。[方法]采用邻二氮菲-Fe2+氧化法、邻苯三酚自氧化法(MTT)和DPPH法分别研究不同体积分数乙醇对虎眼万年青提取物抗氧化活性的影响。[结果]结果表明虎眼万年青乙醇提取物对.OH、O 2-.和DPPH.均有清除作用,且清除能力与其含虎眼万年青总皂苷含量成正比。不同体积分数的虎眼万年青乙醇提取物体外抗氧化能力的大小为70%乙醇提物>80%乙醇提物>60%乙醇提取物>40%乙醇提取物>20%乙醇提取物。[结论]虎眼万年青皂苷类成分与其抗氧化性有关。

[Objective] The study aims to study antioxidant effect of Ornithogalum caudatum Ait of ethanol extracts under different volume fractions in vitro.[Methods] Effects of ethanol with different volume fractions on antioxidant activity of Ornithogalum caudatum Ait are studied by using phenanthroline-Fe2＋ oxidation,pyrogallol autoxidation method（MTT） and DPPH methods.[Results] The results show that ethanol extracts of Ornithogalum caudatum Ait have scavenging on·OH,O-2 · and DPPH·,and scavenging activity is proportional to total saponin content in Ornithogalum caudatum Ait.Moreover,the antioxidant capacity of ethanol extracts under different volume fractions in vitro are 70% ethanol extracts 80% ethanol extracts 60% ethanol extracts 40% ethanol extracts 20% ethanol extracts.[Conclusion] Saponin components have to do with its inoxidizability.