CYP1A1基因多态性与宫颈癌遗传易感性的关系

Relationship between CYP1A1 gene polymorphism and genetic susceptibility of cervical carcinoma

目的探讨CYP1A1基因Exon7位点和Msp1位点多态性与宫颈癌遗传易感性的关系。方法 280例老年宫颈癌患者为实验组,280例健康体检者为对照组,用CR-RELP技术和ASA-PCR技术分别检测两组人群Msp1位点和Exon7位点的多态性。结果两组在CYP1A1Ⅱe/Val位点分布上差异有统计学意义(P<0.05);且携带Ⅱe/Val基因型的个体发生宫颈癌的危险是携带Ⅱe/Ⅱe基因型个体的2.473倍。而两组的Msp1基因型多态性分布频率及OR值均无统计学差异(P>0.05)。结论 CYP1A1Exon7基因多态性是发生宫颈癌的危险因素,有可能成为宫颈癌遗传易感标记物。Msp1位点多态性与宫颈癌易感性可能无关。

Objective To explore the relationship between the polymorphism of CYPlA1 gene and the genetic susceptibility to cervical carcinoma.Methods The polymorphism of CYPlA1 gene at Msp1 and Exon7 locus was detected with CR-RELP and PCR-RFLP in 280 patients with cervical carcinoma（group A） and 280 healthy control subjects（group B）.Results There was a significant difference in the CYP1A1 Exon7 genotype frequencies of Ⅱe/Va between two groups（P〈0.05） and the increaed risk for developing cervical carcinoma in people who carried Ⅱe/Val genetype was 2.473 times as much as that in those who carried Ⅱe/Ⅱe.There was no significant difference in the frequency of CYP1A1 Msp1 between two groups（P〉0.05）.Conclusion The CYP1A1 Exon7 gene polymorphism,but not the CYP1A1 Msp1 gene polymorphism,is the risk factor for cervical carcinoma and could become a marker for genetic susceptibility of cervical carcinoma.