电针刺激对高龄鼠氯胺酮麻醉认知功能及β-淀粉样蛋白抗体的影响

Effect of Electroacupuncture on the Cognitive Function and Plasma Antibodies against Beta-amyloid Protein in Aged Rats with Ketamine Anesthesia

目的研究电针刺激对高龄鼠氯胺酮麻醉后行为学变化以及血清中抗β淀粉样蛋白(Aβ)抗体和海马Aβ的变化,以探索电针对认知功能障碍的影响。方法 14月龄SD大鼠30只,随机分为对照组、氯胺酮麻醉组(麻醉组)和氯胺酮麻醉加电针组(电针组),每组10只。麻醉组和电针组用50mg/kg氯胺酮腹腔注射进行麻醉每天1次,连续7天。电针组从实验第1天开始,待大鼠麻醉完全清醒后,进行电针治疗30min,每日2次,连续7天。Morris水迷宫观测大鼠在原平台象限游泳时间所占比例及逃避潜伏期的变化。实验结束取外周血,采用ELISA法检测血清中抗Aβ抗体含量,采用Western blot方法检测海马组织中Aβ表达。结果高龄鼠应用氯胺酮可以使学习认知能力降低,在定位航行实验中,麻醉组的逃避潜伏期显著延长(P<0.01);空间探索实验中,麻醉组大鼠在原平台象限游泳时间所占总时间比例显著减少(P<0.01)。实验第7天,麻醉组血清抗Aβ抗体含量低于对照组(P<0.05),而电针组大鼠血清中抗Aβ抗体含量显著高于麻醉组(P<0.01)。实验第7天麻醉组海马组织Aβ表达水平高于对照组(P<0.05)。结论电针治疗增加高龄鼠氯胺酮麻醉抗Aβ抗体产生,降低海马组织Aβ的表达,减轻了Aβ的沉积,可以改善大鼠麻醉后认知功能障碍。

Objective To study the effects of electroacupuncture（EA） on the changes of behavior after ketamine anesthesia,and changes of serum antibodies against beta-amyloid（Aβ） and Aβ protein in the hippocampus of aged rats,thus exploring the effects of EA on the cognitive dysfunction. Methods Thirty 14-month old SD rats were randomly divided into 3 groups,i.e.,the control group（Group A） ,the ketamine anesthesia group（Group B） ,and the EA＋ketamine anesthesia group（Group C） ,10 in each group. 50 mg/kg katemine was intraperitoneally injected to rats in Group B and Group C,once daily for 7 successive days. EA was performed to rats in Group C from the 1st day of the experiment after rats awoke completely from anesthesia,twice daily for 7 successive days. Changes of the ratio of the swim time in the original platform quadrant to the total swim time and the escape latency phase were observed by Morris water maze. The peripheral blood was withdrawn by the end of the experiment. Serum anti-Aβ antibody contents were detected using enzyme-linked immunosorbent assay（ELISA） . The expressions of Aβ in the hippocampus were detected using Westen blot. Results Long-term application of ketamine could lower aged rats′ cognitive function. In the navigation test,the escape latency phase of rats in Group B was significantly prolonged（P0.01） . On the 7th day of the experiment,the serum level of anti-Aβ antibodies was lower in Group B than in Group A（P0.05） ,while the serum level of anti-Aβ antibodies was significantly higher in Group C than in Group B（P0.01） . On the 7th day of the experiment,the expression of Aβ in the hippocampus was higher in Group B than in Group A（P0.05） . Conclusion EA could increase the contents of anti-Aβ antibodies in aged rats with ketamine anesthesia,decrease the expression of Aβ in the hippocampus,alleviate the deposition of Aβ,thus improving rats′ cognitive dysfunction.