高尿酸血症大鼠血清尿酸与C反应蛋白关系研究

Relationship between serum uric acid and C-reactive protein in hyperuricemia rats

目的探讨高尿酸血症大鼠血清尿酸和C反应蛋白的关系。方法采用氧嗪酸钾联合酵母膏建立高尿酸血症大鼠模型,24只大鼠随机分为对照组与模型组各12只,分别于第0,2,4,6周检测2组大鼠血清尿酸及C反应蛋白水平,于6周末处死大鼠,观察2组大鼠主动脉内皮组织病理形态学与免疫组织化学染色情况。结果模型组血清C反应蛋白和尿酸水平明显高于对照组(P<0.01);血清尿酸与C反应蛋白呈明显正相关(r=0.830 4,P<0.01);模型组主动脉内皮明显肿胀,主动脉C反应蛋白表达较对照组增强(P<0.01)。结论高尿酸血症大鼠存在炎症反应,可损伤主动脉内皮细胞功能,血尿酸与C反应蛋白表达呈正相关。

Objective To study the relationship between blood serum uric acid and C-reactive protein in hyperuricemia rats. Methods Oxonic acid potassium and yeast extract paste were used to establish hyperuricemia rat models. Twenty rats were randomly divided into model group and control group with 12 rats in each group. The levels of blood uric acid and C-reactive protein were measured in 0, 2, 4 and 6 weeks respectively. In six weeks all rats were executed to observe the pathological change of aorta and immunohistochemical staining of C-reactive protein. Results The levels of serum C reactive protein and uric acid were obviously higher in model group than those in control group（P〈 0.01）. The serum uric acid level was positively correlated with C-reactive protein（r =0. 830 4, P〈0.01）. The aortic endothelium markedly swelled in model group and the expression of aortic C reactive protein was also relatively enhanced compared with that in control group. ConclusionInflammatory reaction in hyperuricemia rats may injure the function of aorta endotheliocyte. Serum uric acid is positively correlated with expression of C reactive protein.