癫癎患儿外周血中调节性T细胞的变化及其临床意义

Changes of regulatory T cells in the peripheral blood of children with epilepsy

目的研究CD4+CD25+Foxp3+调节性T细胞(Treg细胞)在癫癎患儿外周血的表达水平,探讨其在癫癎发病机制中的作用。方法采用流式细胞术分别检测41例癫癎患儿和38例健康儿童外周血Treg细胞占CD4+T细胞的比例,及CD4+T细胞、CD8+T细胞、自然杀伤细胞(NK)、B细胞分别占淋巴细胞的比例。结果癫癎组患儿外周血Treg细胞比例、CD4+T细胞比例、CD4+/CD8+比值分别为(2.4±0.5)%、(35±5)%、1.32±0.24,较对照组的(6.1±1.2)%、(38±4)%、1.60±0.24显著降低(P<0.05);而癫癎组CD8+T细胞比例、NK细胞比例、B淋巴细胞比例分别为(27±3)%、(11.1±5.1)%、(24±9)%,较对照组的(24±3)%、(8.5±1.9)%、(16±5)%显著升高(P<0.05)。结论癫癎患儿外周血中存在Treg细胞比例异常,提示免疫机制可能参与了癫癎的发生和发展。[中国当代儿科杂志,2011,13(11):889-892]

Objective To study the levels of CD4＋ CD25＋ Foxp3＋ regulatory T cells（Tregs） in the peripheral blood of children with epilepsy and the roles of Tregs in the pathogenesis of epilepsy.Methods Forty-one children with epilepsy and thirty-eight healthy children were enrolled.The percentage of CD4＋ CD25＋ Foxp3＋ Tregs in CD4＋ T cells and the percentages of CD4＋ T cells,CD8＋ T cells,nature killer（NK） cells and B cells in lymphocytes were evaluated by flow cytometry.Results The percentages of peripheral blood CD4＋ CD25＋ Foxp3＋ Tregs and CD4＋ T cells and the ratio of CD4＋ T cells to CD8＋ T cells in epileptic children were（2.4±0.5）%,（35±5）% and 1.32±0.24 respectively,which were significantly lower than those in healthy children [（6.1±1.2）%,（38±4）% and 1.60±0.24 respectively;P0.05].In contrast,the percentages of CD8＋ T cells,NK cells and B cells in lymphocytes in epileptic children were significantly higher than those in healthy children [（27±3）% vs（24±3）%,（11.1±5.1）% vs（8.5±1.9）% and（24±9）% vs（16±5）% respectively;P0.05].Conclusions The abnormal percentage of CD4＋ CD25＋ Foxp3＋ Tregs in the peripheral blood may be involved in the pathogenesis of epilepsy.