摘要
目的探讨雪旺细胞(Schwann cells,SCs)在丙烯酰胺(acrylamide,ACR)对运动神经元损伤及修复过程中的作用。方法原代培养大鼠SCs,用插入式培养皿进行SCs与运动神经元VSC4.1的混合培养;噻唑蓝(MTT)比色法检测不同浓度ACR对单独和混合培养运动神经元VSC4.1相对存活率的影响;细胞免疫荧光法检测ACR损伤和修复过程中运动神经元VSC4.1中生长相关蛋白43(growth-associated protein,GAP-43)水平的变化。结果 ACR染毒运动神经元VSC4.1 24 h,单独培养组的IC50为329μg/ml,混合培养的IC50为558μg/ml;GAP-43的水平较对照明显降低,混合培养组GAP-43的平均荧光强度(0.076±0.008)高于单独培养组(0.037±0.013),P<0.05。VSC4.1染毒24 h后去除ACR暴露并继续培养24 h,GAP-43的水平较染毒24 h时上升,混合培养组GAP-43的平均荧光强度值(0.241±0.033)高于单独培养组(0.143±0.034),P<0.05。结论 ACR对运动神经元VSC4.1具有明显的毒性作用,SCs对ACR所致运动神经损伤具有保护作用,同时对损伤后修复过程发挥一定的促进作用。
Objective To explore the effects of Schwan cells(SCs) on damage and repair of motor neuron induced by acrylamide.Methods Primary culture of rat SCs,subculture of VSC4.1 and co-culture of these two kinds of cells were conducted.Cell relative survival rate and changes of GAP-43 were examined by MTT and cellular immunofluorescence method.Results MTT tests showed that IC50 of VSC4.1 treated with acrylamide for 24 h was 329 μg/ml,and for the co-cultured was 558μg/ml.Compared with the non co-cultured VSC4.1,the relative survival rates of co-cultured VSC4.1 were increased in the treatment groups(P0.05).As for the groups treated with ACR for 24 hours,the expression of GAP-43 in both co-cultured groups and non co-cultured groups were decreased compared with the control group.The expression of GAP-43 in co-cultured group was more than that in the non co-cultured group(P0.05).As for groups repair 24 hours after treatment with ACR,the expression of GAP-43 in both co-cultured groups and non co-cultured groups was less than that in the control group but more than groups treated with ACR for 24 hours.The expression of GAP-43 in co-cultured group was more than that in the non co-cultured group(P0.05).Conclusion Acrylamide has obvious toxic effect on motor neuron VSC4.1.SCs might have protective effects on damage induced by acrylamide.And Scs can promote the repair of motor neuron.
出处
《毒理学杂志》
CAS
CSCD
北大核心
2011年第5期328-331,共4页
Journal of Toxicology
基金
国家自然科学基金(30771823)