邻苯基苯酚对HepG2细胞的遗传毒性及氧化应激机制的研究

Possible cytotoxic and genetoxic effects and its mechanism of oxidcotive stress induced by ortho-phenylphenol(OPP) in human HepG2 cells

目的探讨邻苯基苯酚(OPP)的细胞毒性作用及其机制。方法通过体外细胞试验,用OPP作用HepG2细胞,通过彗星试验观察DNA链断裂、测定ROS、GSH水平和抗氧化剂TH干预后ROS、GSH水平变化。结果 OPP能够引起细胞DNA损伤(尾DNA%由空白的0.06增加为800μmol/L OPP的0.98等);细胞内ROS水平增高(P<0.01)和GSH水平降低(P<0.01);TH抗氧化干预效果明显。结论 OPP对HepG2细胞具有遗传毒性,其损伤机制与氧化应激有关。

Objective To assess the cytotoxicity of OPP in HepG2 cells and its mechanism of oxidative stress.Methods DNA damage of HepG2 cells treated with OPP in vitro were detected by the single cell gel electrophoresis assay（SCGE）.The levels of reactive oxygen species（ROS） and glutathione（GSH） were also determined when cells were pretreated with HT and without HT before.Results OPP caused DNA strand breaks（Tail DNA % changed from 0.06 to 0.98 while HepG2 cells treated with OPP of 800μM）;there was an increase in the level of intracellular ROS（P0.01） and a decrease in the level of intracellular GSH（P0.01）;HT had significant inoxidizability.Conclusion OPP could induce genotoxic effects in HepG2 cells and its mechanism of damage might involve in oxidative stress.