中链甘油三酯对C57BL/6J小鼠棕色脂肪组织的影响

Effect of medium chain triglycerides on brown adipose tissue in C57BL/6J mice

目的观察中链甘油三酯(MCT)对C57BL/6J小鼠棕色脂肪组织的影响。方法选择4-5周龄C57BL/6J雄性小鼠30只,随机分为中链甘油三酯高脂组(MCT),大豆油高脂组(LCT)及普通饲料对照组,每组10只,喂饲15周。观察研究前后各组间小鼠体质量、饲料消耗量及食物功效比、棕色脂肪组织重量差异以及解偶联蛋白-1(UCP1)表达水平的变化。结果研究结束时,MCT高脂组小鼠的体质量、体脂肪量显著低于LCT组(P<0.05),饲料消耗量MCT组和LCT组之间无统计学差异(P>0.05),MCT组棕色脂肪组织重量及UCP1蛋白表达量显著高于LCT组。结论 MCT可促进棕色脂肪组织增殖,提高棕色脂肪组织UCP1的表达,增强棕色脂肪组织的产热功能,从而抑制高脂饲料诱发的C57BL/6J小鼠体质量增加和体脂肪积累。

Objective To study the effect of medium chain triglycerides（MCT） on brown adipose tissue（BAT） in C57BL/6J mice.Methods Thirty male C57BL/6J mice at the age 4-5 weeks,enrolled in this study,were randomly divided into MCT group,LCT group and control group（10 in each group）.Animals in the first two groups were fed with high fat diet containing MCT or LCT,and those in control group were fed with standard chow for 15 weeks.Body weight,subscapular brown fat mass,diet consumption,and uncoupled protein-1（UCP1） expression in BAT of different groups were observed.Results After 15 weeks,the body weight and body fat mass were significantly lower in MCT group than in LCT group（P〈0.05）.No significant difference was noted in diet consumption between MCT and LCT groups（P〈0.05）.The brown fat mass and UCP1 expression were significantly higher in MCT group than in LCT group（P〈0.05）.Conclusion MCT can inhibit the increase of body mass and accumulation of body fat due to high fat diet by increasing brown fat mass,UCP1 expression and heat production in brown fat tissue.