HPLC法测定固本咳喘胶囊和补骨脂中补骨脂素和异补骨脂素的含量

Determination of Psoralen and Isopsoralen in Guben Kechuan capsule by HPLC

【目的】采用高效液相色谱法测定固本咳喘胶囊和补骨脂中补骨脂素和异补骨脂素的含量。【方法】用C18柱(4.6 mm×250 mm,5μm),以甲醇:水(52:48)为流动相,检测波长246 nm。【结果】固本咳喘胶囊中补骨脂素平均回收率为96.35%(RSD=0.71%,)异补骨脂素平均回收率为102.8%(RSD=1.30%)。【结论】该方法简便、快速、准确,适用于固本咳喘胶囊和补骨脂的质量控制。

【Objective】The Psoralen and Isopsoralen content in Guben Kechuan capsule and Psoralea corylifolia L.was determined by HPLC..method.【Methods】The chromatographic systems consists of C18 column and mobile phase of CH3OH-H2O-H3PO4（52：48） with a detection wavelength at 246 nm.【Results】Average recovery of Psoralen and Isopsoralen was 96.35%（RSD=0.71%） and 102.8%（RSD=1.30%） respectively.【Conclusion】This method is simply,rapid,accurate and can be used to control the quality of Guben Kechuan capsule and Psoralea herb.