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瞬时受体电位阳离子通道蛋白6合成肽的设计与合成 被引量：3

Design and synthesis of transient receptor potential 6(TRPC6) peptide
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摘要 目的预测瞬时受体电位阳离子通道蛋白6(TRPC6)的抗原表位特性,筛选合适的TRPC6氨基酸序列合成多肽,用于制备抗TRPC6合成肽单克隆抗体。方法根据TRPC6氨基酸序列,采用IEDB分析及蛋白质软件(Lasergene710WinInstall)分析,预测TRPC6表面性、抗原性、亲水性及可能的二级结构性质,以此筛选合成多肽序列。结果确定合成多肽为TRPC6的胞外段565-579氨基酸序列,其表露性预测值1.000;柔韧性预测值1.008;β-转角预测值1.022;抗原性预测1.034;亲水性预测2.043。结论所确定的TRPC6多肽序列表位预测符合要求,为TRPC6合成肽制备抗单克隆抗体提供了一条有效的途径。 Objective Predicting the antigen characteristics of short transient receptor potential channel of protein6（TRPC6） and screening appropriate synthetic polypeptide of TRPC6,in order to prepare the specific anti-TRPC6 monoclonal antibody.Methods Analysis of TRPC6 amino acid sequence by IEDB and Lasergene710WinInstall software,and prediction of epitope,antigenicity,hydrophilia and secondary structure of TRPC6.Results The synthetic polypeptide sequence was determined,which is consisted of 565-579 amino acid of TRPC6 extracellular domain.The surface accessibility,flexibility,beta-turn,antigenicity and hydrophilicity were 1.000,1.008,1.022,1.034 and 2.043,respectively.Conclusion The prediction of TRPC6 synthetic polypeptide meets the requirement for preparing anti-TRPC6 monoclonal antibody.
作者 张萃 梅俪凡 卢文菊 张晨婷 刘晓波 黄超贤
机构地区 广东药学院微生物学与免疫学教研室 中山市博爱医院肾内科 广州医学院第一附属医院呼吸疾病国家重点实验室
出处 《广东药学院学报》 CAS 2011年第5期514-517,共4页 Academic Journal of Guangdong College of Pharmacy
基金 广东省中山市科技计划项目(20102A021) 中山市华南中医药城项目(20101H014)
关键词 TRPC6 合成肽 设计 计算机分析 TRPC6 synthetic polypeptide design computer analysis
分类号 R392.11 [医药卫生—免疫学]
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参考文献5

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二级参考文献42

  • 1高媛,杨金菊,柳晓兰,刘莉,刘超燕,Gian P.Visentin,高建恩,孙启鸿.兔抗人CXCR3-B分子N端多肽多克隆抗体的制备与初步应用[J].细胞与分子免疫学杂志,2006,22(2):223-226. 被引量：6
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共引文献4

同被引文献20

  • 1郑山根,文维延,刘北一,朱平,富宁.模拟鼠伤寒脂多糖表位合成肽的初步研究[J].免疫学杂志,2005,21(3):166-169. 被引量：3
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  • 10ZHU Ting, JI Zhengxiang, XU Caixia, et al. Expression and prognostic role of SGTA in human breast carcinoma correlates with tumor cell proliferation [ J ]. J Mol Histol, 2014,45(6): 665-677.

引证文献3

二级引证文献9

广东药学院学报
2011年 第5期

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