大豆突变体Gm-lpa-TW-1中低植酸性状与种子发芽率和糖份含量的相关性分析

RELATIONSHIP OF LOW PHYTATE TRAIT WITH SEED GERMINATION AND CARBOHYDRATES CONTENT IN SOYBEAN MUTANT Gm-lpa-TW-1

为明确低植酸突变体Gm-lpa-TW-1植酸性状突变与种子发芽率降低以及糖份含量变化的相关性,选用低植酸突变体Gm-lpa-TW-1与不同野生型亲本的杂交后代,低植酸纯合株系(LPA)和非低植酸纯合株系(HPA)为研究材料,分析不同株系在不同种植环境下收获种子的发芽率、总糖、蔗糖和低聚糖的含量。结果表明:无论是LPA株系还是HPA株系春播种子的发芽率均显著低于秋播种子;同一杂交组合中LPA株系春播种子的发芽率显著低于HPA株系春播种子;鲜食大豆杂交组合后代的LPA株系发芽率低于粒用大豆杂交组合的LPA株系;但在所有杂交组合后代株系中均可以筛选到发芽率显著高于突变亲本Gm-lpa-TW-1的LPA株系。糖份含量分析表明:LPA和HPA株系之间总糖含量无明显差异;LPA株系的蔗糖含量极显著地高于HPA株系的蔗糖含量,低聚糖含量则表现为极显著低于HPA株系,3种糖份含量在LPA和HPA株系之间的差异不受种植环境影响。LPA株系种子发芽率受到突变基因与环境互作的影响,高温环境对LPA株系种子的发芽率影响较大;通过遗传改良可以有效改善LPA突变基因对种子发芽率的不良影响。LPA株系中的低植酸性状与高蔗糖低寡聚糖性状表现为连锁遗传,有利于选育品质更为优良的大豆新品种。

The relationship between the low phytate mutation with seed germination and carbohydrate content homozygous F5 lines with/without lpagene derived from mutant and different wild type parents were analyzed.The results showed that LPA（low phytic acid）/HPA（high phytic aicd） lines developed in autumn had higher seed germination rate than that developed in spring.LPA lines had lower seed germination rate than HPA lines when they all developed in spring.However,in all crosses LPA lines with higher seed germination rate than mutant parent Gm-lpa-TW-1 was observed.No significant difference was detected between LPA and HPA lines when they developed in autumn.Homozygous LPA lines derived from vegetable soybean had lower seed germination than those from non-vegetable soybean varieties.There was no significant difference in total carbohydrate content between LPA and HPA homozygous lines,but the sugar content of LPA homozygous lines was significant higher than HPA lines.On the contrary,oligosaccharides content with LPA lines were significant lower than those with HPA homozygous lines in all planting environment.It is concluded that seeds field germination rate were affected by low phytate mutation gene and planting environment,and lower seed germination rate phenotype of LPA lines could be improved by genetic method.Mutant Gm-lpa-TW-1 of LPA phenotype was genetic linkage with high sugar and low oligosaccharides phenotype,so it should be benefit to breed new soybean varieties with better quality.