Cry1Ac蛋白降解菌株FJSB3的分离鉴定及降解特性

ISOLATION AND IDENTIFICATION OF Cry1Ac PROTEIN-DEGRADING BACTERIA AND ITS DEGRADATION CHARACTERISTICS

从转Cry1Ac基因水稻种植田土壤中,分离纯化得到1株能高效降解Cry1Ac蛋白的细菌FJSB3。通过表型特征、16S rDNA扩增和电镜观察,初步鉴定FJSB3为寡养单胞菌(Stenotrophomonas sp.)。FJSB3发酵液能降解粗Bt蛋白。通过单因素试验确定FJSB3降解水稻秸秆中Cry1Ac蛋白的最适条件为:发酵温度30℃,初始菌液浓度2.0×106CFU/ml,pH 7.0,碳氮比(C/N)25/1。在此条件下,水稻秸秆中Cry1Ac蛋白降解率达到92.86%。

A degradation of Cry1Ac protein in rice straw,bacterium FJSB3 capable of highly degrading Cry1Ac protein was isolated from the agricultural soil where the transgenic Cry1Acrice was grown.The FJSB3 was identified preliminarily as Stenotrophomonas sp.by phenotype observation,16S rDNA analysis and electron microscope.SDS-PAGE showed that FJSB3 could degrade Btprotein.Based on the single factor experiments,the optimal conditions of degrading Cry1Ac protein in rice straw were： 30℃,initial concentration of bacterium 2.0×106 CFU/ml,pH 7.0,C/N 25/1.Under these conditions,the degradation rate of Cry1Ac protein reached 92.86%.