芥菜Rubisco小亚基的基因克隆及其在芜菁花叶病毒侵染后的表达分析

CLONING OF RUBISCO SMALL SUBUNIT GENE FROM MUSTARD AND ANALYSIS OF ITS EXPRESSION IN RESPONSE TO TURNIP MOSAIC VIRUS INFECTION

以耐病性叶用芥菜为材料,克隆和分析了芥菜Rubicso小亚基的基因序列,研究了芜菁花叶病毒(Turnip mosaic virus,TuMV)侵染对Rubicso小亚基基因表达的影响。结果显示:Rubicso小亚基基因全长为842bp,其开放阅读框(ORF)可编码181个氨基酸,其中包括2个α螺旋及4个β折叠二级结构,并含有一些高度保守的氨基酸残基。接种TuMV后,随着上部第3片真叶中外壳蛋白基因的逐渐积累,叶片的正常生长受到抑制,同时Rubicso小亚基的表达呈现先上升后下降的趋势,表明其参与了芥菜对TuMV侵染的响应。

We cloned the cDNA encoding the Rubisco small subunit of leafy mustard and studied its expression after Turnip mosaic virus（TuMV） infection.The result showed that the full-length cDNA of Rubisco small subunit gene was 842bp and contained an open reading frame（ORF） encoding 181 amino acids,which had two α-helixes,four β-sheets,and some conserved residues.With the accumulation of CP in upper systemic leaves after TuMV infection,mustard growth was impaired.At the same time,expression of Rubisco small subunit was increased before 6 days of inoculation and then gradually decreased,which suggested that the Rubisco small subunit participated in the response of mustard to TuMV infection.