湖北荆门地区RhD阴性无偿献血者表型分布调查

RhD Negative Phenotype Distribution Investigation of Unpaid Blood Donors in Hubei Jingmen Region

目的 了解湖北荆门地区无偿献血者中RhD阴性表型分布情况.方法 常规血清学方法初筛RhD阴性标本,用间接抗球蛋白试验确认弱D,用吸收放散试验检测Del,RhC,c,E和e抗原使用血清学方法进行检测.结果 38 921名无偿献血者中初筛为RhD阴性124例;用抗人球蛋白试验确认弱D 11例;除11例弱D和10例假阴性后,103例确认为Rh阴性标本中共检出Del型25例;103例确认为Rh阴性的标本,血清学表型分布为dccee 63例,dCcee 28例,dCCee 6例,dccEe 4例,dccEE 1例,dCcEe 1例.结论 湖北荆门地区RhD阴性献血者中存在相当比例人群携带弱D表达抗原,为确保临床用血的安全,应加强弱D或Del型的确认.

Objective To understand the Hubei blood donors in Jingmen area of RhD negative phenotype distribution. Methods Screened early RhD negative specimens with routine serologic. Used indirect antiglobulin test to confirm the weak D. Detected Del,RhC,C and E with absorption elution test ,and used serological methods to detect e-antigen. Results 38 921 volunteer blood donors in screening for RhD negative had 124 cases. Using antiglobulin test to confirm the weak D had 11 cases. In addition to 11 weak D and 10 false negative,25 Del type was detected in 103 Rh negative specimens. In 103 Rh negative specimens,serological phenotype distribution were dccee for 63 patients ,dCcee for 28 patients ,dCCee for 6 patients,dccEe for 4 patients,dccEE for 1 patients,dCcEe for 1 patients. Conclusion RhD negative blood donors in Hubei area of Jingmen in the presence of a considerable proportion of population carryied weak expression of D antigen. In order to ensure the safety of blood for clinical use,we should strengthen the weak D or Del type recognition.